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• W2033528405 abstract "The aim of this study was to investigate the feasibility of nuclear molecular imaging using the human sodium iodide symporter (hNIS) as a reporter gene to monitor macrophage migration toward the inflammatory foci. <b>Methods:</b> A stable macrophage cell line coexpressing hNIS and green fluorescent protein (GFP) genes (RAW264.7/hNIS-GFP and R_NIS cell) was established from an immortalized macrophage cell line (RAW264.7 cells). ¹²⁵I uptake was determined (for hNIS protein functional activity), and flow cytometry analysis (to examine GFP gene expression), a cell proliferation assay, a cytokine assay, and a phagocytic activity assay were performed. ^99mTc-pertechnetate images were acquired at 1 d after subcutaneous inoculation of R_NIS cells in nude mice. Chemical inflammation was induced for in vivo imaging in the thigh of nude mice by turpentine oil injection. Small-animal PET with ¹⁸F-FDG and ¹²⁴I was performed with an intravenous administration of RAW264.7 or R_NIS cells in inflammation-induced animals. <b>Results:</b> The expression of hNIS and GFP genes was confirmed in R_NIS cells by flow cytometry and immunofluorescent staining. ¹²⁵I uptake was about 67 times higher in R_NIS cells than in RAW264.7 cells. No significant difference was observed in cell proliferation, cytokine production, and phagocytic activity between RAW264.7 and R_NIS cells. ^99mTc-pertechnetate imaging revealed increased tracer uptake at the inoculation site. PET with ¹²⁴I demonstrated a donut-shaped uptake, correlating with uptake shown by the ¹⁸F-FDG PET images, at the inflammation site of mice administered R_NIS cells. ¹²⁴I uptake (percentage injected dose per gram) was about 2.12 times higher at the inflammation site in the R_NIS mice than in RAW264.7 mice. By immunohistochemistry, the migration of macrophages was further confirmed by positive staining for GFP and hNIS at the inflammation site of R_NIS mice. <b>Conclusion:</b> These data support the feasibility of hNIS reporter gene imaging to monitor the macrophage migration toward an inflammatory lesion. Macrophages expressing hNIS may provide a new strategy to investigate the cellular behavior seen with inflammatory response in a preclinical model." @default.
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• W2033528405 date "2010-09-16" @default.
• W2033528405 modified "2023-09-25" @default.
• W2033528405 title "Trafficking Macrophage Migration Using Reporter Gene Imaging with Human Sodium Iodide Symporter in Animal Models of Inflammation" @default.
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• W2033528405 doi "https://doi.org/10.2967/jnumed.110.077891" @default.
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