FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033530021> ?p ?o ?g. }

• W2033530021 endingPage "255" @default.
• W2033530021 startingPage "243" @default.
• W2033530021 abstract "Very high cell densities and optimal vascularization characterize among others organs and tissues in vivo. In order to study organ-specific functions in vitro or to make use of them in medical devices/treatments in the future, this natural architecture should be rebuilt. An important aspect in this context is the appropriate ratio of medium to cell volume being so far not optimally reestablished in most of the currently available in vitro systems. To improve such culture conditions, we constructed a microstructure to culture hepatocytes and (without any addition of extracellular matrix material) characterized liver tissue in the form of evenly sized aggregates. The liver-specific differentiation status of such aggregates was monitored by their ability to perform CYP450 dependent xenobiotic metabolism along with the measurement of albumin secretion. Freshly isolated adult rat hepatocytes show an initial loss of total CYP450 content and of associated activities (mixed function oxidases). However, in the aggregate system, this level did not decrease further but remained stable or even increased throughout the culture period of 10-13 days. The CYP450 dependent metabolism of the hepatocytes is able to respond to classic inducing agents. The described culture efficiently supports liver-specific functions of adult rat hepatocytes and seems to be suited not only for use in an extracorporeal liver device but also for the formation of evenly sized small aggregates to be of use in transplantation of differentiated liver tissue. Moreover, after design variations, the microstructure can be applied for functional analysis of metabolically active hepatocytes as well as for toxicological and pharmacological validation." @default.
• W2033530021 created "2016-06-24" @default.
• W2033530021 creator A5007431904 @default.
• W2033530021 creator A5011661053 @default.
• W2033530021 creator A5015815782 @default.
• W2033530021 creator A5058202294 @default.
• W2033530021 creator A5062291850 @default.
• W2033530021 creator A5072422348 @default.
• W2033530021 creator A5090821485 @default.
• W2033530021 date "2005-03-15" @default.
• W2033530021 modified "2023-10-09" @default.
• W2033530021 title "Microstructured scaffolds for liver tissue cultures of high cell density: Morphological and biochemical characterization of tissue aggregates" @default.
• W2033530021 cites W1565526431 @default.
• W2033530021 cites W1605194916 @default.
• W2033530021 cites W1698518057 @default.
• W2033530021 cites W1754108643 @default.
• W2033530021 cites W1818065750 @default.
• W2033530021 cites W1868334643 @default.
• W2033530021 cites W1951469402 @default.
• W2033530021 cites W1980460031 @default.
• W2033530021 cites W1994273373 @default.
• W2033530021 cites W2001164956 @default.
• W2033530021 cites W2007297144 @default.
• W2033530021 cites W2007802831 @default.
• W2033530021 cites W2008419654 @default.
• W2033530021 cites W2016955856 @default.
• W2033530021 cites W2022978909 @default.
• W2033530021 cites W2028925255 @default.
• W2033530021 cites W2033764551 @default.
• W2033530021 cites W2035172806 @default.
• W2033530021 cites W2038320302 @default.
• W2033530021 cites W2043516503 @default.
• W2033530021 cites W2045257705 @default.
• W2033530021 cites W2062494393 @default.
• W2033530021 cites W2071369430 @default.
• W2033530021 cites W2072045976 @default.
• W2033530021 cites W2072482644 @default.
• W2033530021 cites W2074882843 @default.
• W2033530021 cites W2088227793 @default.
• W2033530021 cites W2090873057 @default.
• W2033530021 cites W2107115514 @default.
• W2033530021 cites W2116440470 @default.
• W2033530021 cites W2149708086 @default.
• W2033530021 cites W2165236937 @default.
• W2033530021 cites W2345297806 @default.
• W2033530021 cites W2411985659 @default.
• W2033530021 doi "https://doi.org/10.1002/jcb.20360" @default.
• W2033530021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15770659" @default.
• W2033530021 hasPublicationYear "2005" @default.
• W2033530021 type Work @default.
• W2033530021 sameAs 2033530021 @default.
• W2033530021 citedByCount "52" @default.
• W2033530021 countsByYear W20335300212012 @default.
• W2033530021 countsByYear W20335300212013 @default.
• W2033530021 countsByYear W20335300212014 @default.
• W2033530021 countsByYear W20335300212015 @default.
• W2033530021 countsByYear W20335300212016 @default.
• W2033530021 countsByYear W20335300212017 @default.
• W2033530021 countsByYear W20335300212019 @default.
• W2033530021 crossrefType "journal-article" @default.
• W2033530021 hasAuthorship W2033530021A5007431904 @default.
• W2033530021 hasAuthorship W2033530021A5011661053 @default.
• W2033530021 hasAuthorship W2033530021A5015815782 @default.
• W2033530021 hasAuthorship W2033530021A5058202294 @default.
• W2033530021 hasAuthorship W2033530021A5062291850 @default.
• W2033530021 hasAuthorship W2033530021A5072422348 @default.
• W2033530021 hasAuthorship W2033530021A5090821485 @default.
• W2033530021 hasConcept C126322002 @default.
• W2033530021 hasConcept C1491633281 @default.
• W2033530021 hasConcept C150903083 @default.
• W2033530021 hasConcept C151730666 @default.
• W2033530021 hasConcept C189165786 @default.
• W2033530021 hasConcept C202751555 @default.
• W2033530021 hasConcept C207001950 @default.
• W2033530021 hasConcept C2776125364 @default.
• W2033530021 hasConcept C2776200302 @default.
• W2033530021 hasConcept C2779343474 @default.
• W2033530021 hasConcept C28406088 @default.
• W2033530021 hasConcept C2911091166 @default.
• W2033530021 hasConcept C2992672570 @default.
• W2033530021 hasConcept C49039625 @default.
• W2033530021 hasConcept C54355233 @default.
• W2033530021 hasConcept C55493867 @default.
• W2033530021 hasConcept C62231903 @default.
• W2033530021 hasConcept C71924100 @default.
• W2033530021 hasConcept C81885089 @default.
• W2033530021 hasConcept C86803240 @default.
• W2033530021 hasConcept C95444343 @default.
• W2033530021 hasConceptScore W2033530021C126322002 @default.
• W2033530021 hasConceptScore W2033530021C1491633281 @default.
• W2033530021 hasConceptScore W2033530021C150903083 @default.
• W2033530021 hasConceptScore W2033530021C151730666 @default.
• W2033530021 hasConceptScore W2033530021C189165786 @default.
• W2033530021 hasConceptScore W2033530021C202751555 @default.
• W2033530021 hasConceptScore W2033530021C207001950 @default.
• W2033530021 hasConceptScore W2033530021C2776125364 @default.
• W2033530021 hasConceptScore W2033530021C2776200302 @default.
• W2033530021 hasConceptScore W2033530021C2779343474 @default.

• next