Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033532181> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2033532181 abstract "Plasmids carrying three types of TEM-type extended-spectrum beta-lactamase (ESBL) genes, encoding TEM-3, TEM-5, and TEM-9, respectively, were constructed by site-directed mutagenesis. ESBL producers were prepared by transformation of Escherichia coli JM109 with a plasmid carrying one gene of either the three TEM types, an SHV-type, or a Toho-1 group gene. This strategy with the same vector and host strain can exclude the contribution of other factors to susceptibility, and is useful in Japan, where few TEM-type ESBL producers have been isolated. In vitro antibacterial activities of 23 beta-lactam antibiotics were tested against the ESBL producers by the agar dilution method, and the results were compared. The minimum inhibitory concentrations (MICs) of penicillins tested were more than 32 micro g/ml against both the parental RTEM and ESBL producers, but they were substantially decreased by a combination with beta-lactamase inhibitors. Compared with the MICs against the ESBL-nonproducing host strain, the MICs of the cephalosporins tested for the ESBL producers were increased more than eight times in most cases and in several cases soared to more than 2048 times against a Toho-1 ESBL producer. On the other hand, the MICs of carbapenem, cephamycin, and penem antibiotics were generally comparable to those against the host strain, and were increased by 32 times at most. Kinetic analysis revealed that extended-spectrum cephalosporins were hydrolyzed only slightly to moderately by the TEM-type ESBLs, while carbapenems and a cephamycin were scarcely hydrolyzed, and rather inhibited or inactivated the mutant enzymes." @default.
- W2033532181 created "2016-06-24" @default.
- W2033532181 creator A5036891979 @default.
- W2033532181 creator A5044049628 @default.
- W2033532181 creator A5056798404 @default.
- W2033532181 date "2002-01-01" @default.
- W2033532181 modified "2023-09-27" @default.
- W2033532181 title "Properties of extended-spectrum β-lactamases constructed by site-directed mutagenesis" @default.
- W2033532181 cites W1539678687 @default.
- W2033532181 cites W1601568241 @default.
- W2033532181 cites W1913562629 @default.
- W2033532181 cites W2028622989 @default.
- W2033532181 cites W2040198458 @default.
- W2033532181 cites W2044000162 @default.
- W2033532181 cites W2077360598 @default.
- W2033532181 cites W2078340635 @default.
- W2033532181 cites W2115255353 @default.
- W2033532181 cites W2119630062 @default.
- W2033532181 cites W2127732987 @default.
- W2033532181 cites W2134352676 @default.
- W2033532181 cites W2146993729 @default.
- W2033532181 cites W2150271126 @default.
- W2033532181 cites W2161985926 @default.
- W2033532181 cites W2163823303 @default.
- W2033532181 doi "https://doi.org/10.1007/s10156-002-0183-9" @default.
- W2033532181 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12373483" @default.
- W2033532181 hasPublicationYear "2002" @default.
- W2033532181 type Work @default.
- W2033532181 sameAs 2033532181 @default.
- W2033532181 citedByCount "3" @default.
- W2033532181 countsByYear W20335321812021 @default.
- W2033532181 crossrefType "journal-article" @default.
- W2033532181 hasAuthorship W2033532181A5036891979 @default.
- W2033532181 hasAuthorship W2033532181A5044049628 @default.
- W2033532181 hasAuthorship W2033532181A5056798404 @default.
- W2033532181 hasConcept C104317684 @default.
- W2033532181 hasConcept C105702510 @default.
- W2033532181 hasConcept C143065580 @default.
- W2033532181 hasConcept C16318435 @default.
- W2033532181 hasConcept C176947019 @default.
- W2033532181 hasConcept C204241405 @default.
- W2033532181 hasConcept C22744801 @default.
- W2033532181 hasConcept C2776685102 @default.
- W2033532181 hasConcept C2778022156 @default.
- W2033532181 hasConcept C2780493024 @default.
- W2033532181 hasConcept C501593827 @default.
- W2033532181 hasConcept C54355233 @default.
- W2033532181 hasConcept C64778159 @default.
- W2033532181 hasConcept C86803240 @default.
- W2033532181 hasConcept C89423630 @default.
- W2033532181 hasConceptScore W2033532181C104317684 @default.
- W2033532181 hasConceptScore W2033532181C105702510 @default.
- W2033532181 hasConceptScore W2033532181C143065580 @default.
- W2033532181 hasConceptScore W2033532181C16318435 @default.
- W2033532181 hasConceptScore W2033532181C176947019 @default.
- W2033532181 hasConceptScore W2033532181C204241405 @default.
- W2033532181 hasConceptScore W2033532181C22744801 @default.
- W2033532181 hasConceptScore W2033532181C2776685102 @default.
- W2033532181 hasConceptScore W2033532181C2778022156 @default.
- W2033532181 hasConceptScore W2033532181C2780493024 @default.
- W2033532181 hasConceptScore W2033532181C501593827 @default.
- W2033532181 hasConceptScore W2033532181C54355233 @default.
- W2033532181 hasConceptScore W2033532181C64778159 @default.
- W2033532181 hasConceptScore W2033532181C86803240 @default.
- W2033532181 hasConceptScore W2033532181C89423630 @default.
- W2033532181 hasLocation W20335321811 @default.
- W2033532181 hasLocation W20335321812 @default.
- W2033532181 hasOpenAccess W2033532181 @default.
- W2033532181 hasPrimaryLocation W20335321811 @default.
- W2033532181 hasRelatedWork W1604203572 @default.
- W2033532181 hasRelatedWork W1921319550 @default.
- W2033532181 hasRelatedWork W1963861764 @default.
- W2033532181 hasRelatedWork W1996456844 @default.
- W2033532181 hasRelatedWork W2006642860 @default.
- W2033532181 hasRelatedWork W2030050275 @default.
- W2033532181 hasRelatedWork W2046318364 @default.
- W2033532181 hasRelatedWork W2067895776 @default.
- W2033532181 hasRelatedWork W2070313642 @default.
- W2033532181 hasRelatedWork W2078795727 @default.
- W2033532181 hasRelatedWork W2099511328 @default.
- W2033532181 hasRelatedWork W2107500510 @default.
- W2033532181 hasRelatedWork W2114195078 @default.
- W2033532181 hasRelatedWork W2115969071 @default.
- W2033532181 hasRelatedWork W2124304820 @default.
- W2033532181 hasRelatedWork W2144729514 @default.
- W2033532181 hasRelatedWork W2146114359 @default.
- W2033532181 hasRelatedWork W2152636222 @default.
- W2033532181 hasRelatedWork W2160418763 @default.
- W2033532181 hasRelatedWork W2167065215 @default.
- W2033532181 isParatext "false" @default.
- W2033532181 isRetracted "false" @default.
- W2033532181 magId "2033532181" @default.
- W2033532181 workType "article" @default.