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• W2033537251 abstract "Determinar las frecuencias de pérdidas de heterocigocidad de LOH en las regiones 6p21.3 y 15q21 que codifican para HLA y β 2 microglobulina, para establecer su correlación con el estadio tumoral, teniendo en cuenta que las LOH en HLA I ocurren como un evento genético temprano del cáncer y pueden contribuir a su desarrollo. Se tomaron muestras de sangre periférica (SP) y biopsias de cuello uterino de pacientes con NICIII y CCU. Se amplificaron 11 microsatélites relacionados con el sistema HLA en pares normal-tumor a partir de ADN purificado de células de SP y células tumorales microdisectadas. Las LOH fueron determinadas por electroforesis capilar y analizadas mediante los programas GeneScan y Genotyper. Todas las muestras amplificaron más de 7 microsatélites (promedio 9,5). El porcentaje de heterocigocidad para los marcadores de microsatélites utilizados en las muestras de cuello uterino varió entre 51,8% y 95%, y la LOH, entre 17,4% y 50,0%. Las frecuencias observadas para LOH en los diferentes estadios tumorales fueron: 42,9% en el grupo de NIC-III; 57% en CCU en estadio I; 63,6% en CCU en estadio II y 92,85% en pacientes con estadios más avanzados (III-IV). Se observó una mayor frecuencia de LOH en los grupos de pacientes con estadios avanzados de CCU, al comparar con pacientes con NIC-III. To determine the frequency of LOH heterozygosity in the regions 6p21.3 and 15q21 which encode for HLA and β2microglobulina in order to establish their correlation with tumoral stage, taking into account that LOH and HLA I occur as an early genetic event in cancer and can contribute to its development. Peripheral blood (PB) samples and cervical biopsies were taken from patients with CIN III and invasive cancer. Amplification was made of eleven microsatellites related to the HLA system, in normal-tumor pairs, based on purified DNA PB cells and micro-dissected tumor cells. LOH were determined through capillary electrophoresis and analyzed with GeneScan and Genotyper programs. All samples amplified at more than 7 microsatellites (average 9.5). The percentage of heterozygosity for microsatellite markers used in the cervical samples varied between 51.8% and 95%; the LOH, between 17.4% and 50.0%. The frequencies observed for LOH in the different tumoral stages were: 42.9% in the CIN III group; 57% in invasive cancer Stage I; 63.6% in Stage II, and 92.85% in patients in the most advanced stages (III-IV). Greater frequency of LOH was observed in groups of patients with advanced stages of invasive cancer in comparison with patients with CIN III." @default.
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• W2033537251 date "2009-01-01" @default.
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• W2033537251 title "Frecuencias de las pérdidas de heterocigocidad en la región que codifica para HLA en biopsias de pacientes con cáncer de cuello uterino" @default.
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