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- W2033584972 abstract "Abstract Preparative high speed countercurrent chromatography (HSCCC) has been successfully used for the isolation and purification of isorhamnetin from Stigma maydis. This was achieved in two stages: the first separation was performed with a two phase solvent system composed of n-hexane-ethyl acetate-methanol-water (HEMW) at a volume ratio of 5:5:5:5, yielding isorhamnetin at 65.6%, which is followed by the second run using a two phase solvent system composed of HEMW 5:5:6:4, v/v. From 700 mg of the crude extract, 11.8 mg of isorhamnetin was obtained at a high purity of 98%. The final identification was performed by MS, 1H-NMR and 13C-NMR spectra." @default.
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- W2033584972 date "2008-12-30" @default.
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- W2033584972 title "Preparative Isolation of Isorhamnetin from <i>Stigma Maydis</i> using High Speed Countercurrent Chromatography" @default.
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- W2033584972 doi "https://doi.org/10.1080/10826070802603369" @default.
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