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- W2033622011 abstract "Fasciola hepatica, hem hayvanlari hem de insanlari enfekte eden, onemli patolojilere ve ekonomik kayiplara sebep olan bir karaciger trematodur. Gunumuze kadar daha cok morfolojik olarak tanimlanan parazit, son yillarda ara Fasciola formlarinin bulunmasiyla birlikte molekuler olarak karakterize edilmeye calisilmistir. Ulkemizde hem hayvanlarda hem de insanlarda saptanan F. hepatica’nin hayvan izolatlari molekuler olarak tanimlanmasina ragmen bugune kadar insan izolatlarinin molekuler karakterizasyonu yapilmamistir. Bu calismada F. hepatica insan izolatinin molekuler karakterizasyonu amaclanmistir. Bu amacla; Kayseri’de insan safra yollarindan cikarilan eriskin F. hepatica’dan elde edilen genomik DNA’dan PCR ile ITS-2 bolgesine spesifik primerler kullanilarak PCR urunu elde edilmistir. Agaroz jelde goruntulenen DNA, jelden Wizard SV Gel and PCR Clean-Up System kullanilarak saflastirilmis ve DNA dizi analizi yaptirilmistir. DNA dizi analiz sonucu, BLAST analizi ve MEGA program ile filogenetik analiz yapilarak degerlendirilmistir. Genomik DNA’dan 510 bp’lik PCR urunu elde edilmistir. DNA dizisi saptanan insan F. hepatica izolatinin, BLAST ve filogenetik analiz sonucunda Turkiye’den bildirilen hayvan izolatlariyla benzer oldugu belirlenmistir. Fazla sayida insan ve hayvan F. hepatica izolatlariyla ve ITS2 disinda diger genetik markerlerle desteklenmesi gereken bu calisma sonucunda; Turkiye’de ilk kez bir insan F. hepatica izolatinin molekuler karakterizasyonu yapilmistir." @default.
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- W2033622011 date "2009-01-01" @default.
- W2033622011 modified "2023-10-17" @default.
- W2033622011 title "Molecular Characterization of Human Isolate of Fasciola hepatica in Turkey" @default.
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- W2033622011 doi "https://doi.org/10.9775/kvfd.2012.6064" @default.
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