FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033626471> ?p ?o ?g. }

• W2033626471 endingPage "352" @default.
• W2033626471 startingPage "345" @default.
• W2033626471 abstract "TSH is a glycoprotein hormone whose carbohydrate content varies among different species. Although recent studies suggest that variants of TSH deficient in carbohydrate occur naturally, the significance of the carbohydrate moiety of TSH in respect to its thyrotropic function is unclear. The present studies were undertaken, therefore, to examine this question. A highly purified preparation of bovine TSH (bTSH) was deglycosylated by treatment with anhydrous hydrogen fluoride. Amino acid and carbohydrate analyses of the original and deglycosylated preparations indicated that approximately 85% of the carbohydrate originally present had been removed and that the protein moiety was unaltered. As judged from TSH radioreceptor assays, bTSH and deglycosylated bTSH (dg-bTSH) bound to human thyroid membranes with equal affinity, since both caused a half-maximal inhibition of [125I]bTSH binding at approximately equal concentrations. Nonetheless, dg-bTSH at optimal concentration displayed only about one third the activity of intact TSH in stimulating adenylate cyclase activity in human thyroid membranes. dg-bTSH also antagonized the adenylate cyclase-stimulating activity of intact bTSH in this system, but only weakly, since abolition of the bTSH effect required an approximately 40-fold higher concentration of dg-bTSH. In cultures of FRTL5 cells, a cloned line of follicular cells derived from normal rat thyroid, both intact and dg-bTSH enhanced cell growth, as measured by [3H]thymidine incorporation and stimulated cAMP release in the medium, but the response elicited by dg-bTSH was much less than that caused by equal concentrations of the intact hormone. In accord with the findings in the in vitro assays, dg-bTSH evoked a much smaller response than bTSH did in the in vivo mouse assay. It is concluded that although not required for receptor recognition, the carbohydrate moiety of bTSH is essential for the full expression of its biological activity. (Endocrinology120: 345–352, 1987)" @default.
• W2033626471 created "2016-06-24" @default.
• W2033626471 creator A5020110509 @default.
• W2033626471 creator A5058677280 @default.
• W2033626471 creator A5070334494 @default.
• W2033626471 creator A5071042751 @default.
• W2033626471 creator A5091065594 @default.
• W2033626471 date "1987-01-01" @default.
• W2033626471 modified "2023-10-02" @default.
• W2033626471 title "The Carbohydrate Moiety of Bovine Thyrotropin Is Essential for Full Bioactivity but Not for Receptor Recognition*" @default.
• W2033626471 cites W105714273 @default.
• W2033626471 cites W1526909016 @default.
• W2033626471 cites W1548637624 @default.
• W2033626471 cites W1568537742 @default.
• W2033626471 cites W1589373559 @default.
• W2033626471 cites W1679692751 @default.
• W2033626471 cites W1775749144 @default.
• W2033626471 cites W191249933 @default.
• W2033626471 cites W1966374416 @default.
• W2033626471 cites W1970198468 @default.
• W2033626471 cites W1987220066 @default.
• W2033626471 cites W1990738136 @default.
• W2033626471 cites W2013588383 @default.
• W2033626471 cites W2028528771 @default.
• W2033626471 cites W2030092770 @default.
• W2033626471 cites W2031402418 @default.
• W2033626471 cites W2044897122 @default.
• W2033626471 cites W2059926505 @default.
• W2033626471 cites W2060641682 @default.
• W2033626471 cites W2064330356 @default.
• W2033626471 cites W2069159786 @default.
• W2033626471 cites W2085474307 @default.
• W2033626471 cites W2092771790 @default.
• W2033626471 cites W2101417661 @default.
• W2033626471 cites W2102876680 @default.
• W2033626471 cites W2164616699 @default.
• W2033626471 cites W2175128997 @default.
• W2033626471 cites W2491003065 @default.
• W2033626471 cites W963971915 @default.
• W2033626471 doi "https://doi.org/10.1210/endo-120-1-345" @default.
• W2033626471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3023032" @default.
• W2033626471 hasPublicationYear "1987" @default.
• W2033626471 type Work @default.
• W2033626471 sameAs 2033626471 @default.
• W2033626471 citedByCount "31" @default.
• W2033626471 countsByYear W20336264712014 @default.
• W2033626471 countsByYear W20336264712015 @default.
• W2033626471 countsByYear W20336264712016 @default.
• W2033626471 countsByYear W20336264712023 @default.
• W2033626471 crossrefType "journal-article" @default.
• W2033626471 hasAuthorship W2033626471A5020110509 @default.
• W2033626471 hasAuthorship W2033626471A5058677280 @default.
• W2033626471 hasAuthorship W2033626471A5070334494 @default.
• W2033626471 hasAuthorship W2033626471A5071042751 @default.
• W2033626471 hasAuthorship W2033626471A5091065594 @default.
• W2033626471 hasConcept C126322002 @default.
• W2033626471 hasConcept C134018914 @default.
• W2033626471 hasConcept C170493617 @default.
• W2033626471 hasConcept C185592680 @default.
• W2033626471 hasConcept C2778977261 @default.
• W2033626471 hasConcept C2780069284 @default.
• W2033626471 hasConcept C71924100 @default.
• W2033626471 hasConcept C86803240 @default.
• W2033626471 hasConceptScore W2033626471C126322002 @default.
• W2033626471 hasConceptScore W2033626471C134018914 @default.
• W2033626471 hasConceptScore W2033626471C170493617 @default.
• W2033626471 hasConceptScore W2033626471C185592680 @default.
• W2033626471 hasConceptScore W2033626471C2778977261 @default.
• W2033626471 hasConceptScore W2033626471C2780069284 @default.
• W2033626471 hasConceptScore W2033626471C71924100 @default.
• W2033626471 hasConceptScore W2033626471C86803240 @default.
• W2033626471 hasIssue "1" @default.
• W2033626471 hasLocation W20336264711 @default.
• W2033626471 hasLocation W20336264712 @default.
• W2033626471 hasOpenAccess W2033626471 @default.
• W2033626471 hasPrimaryLocation W20336264711 @default.
• W2033626471 hasRelatedWork W1981780460 @default.
• W2033626471 hasRelatedWork W2029369136 @default.
• W2033626471 hasRelatedWork W2033472167 @default.
• W2033626471 hasRelatedWork W2034583712 @default.
• W2033626471 hasRelatedWork W2056306087 @default.
• W2033626471 hasRelatedWork W2063619316 @default.
• W2033626471 hasRelatedWork W2155585839 @default.
• W2033626471 hasRelatedWork W2169225653 @default.
• W2033626471 hasRelatedWork W2398181117 @default.
• W2033626471 hasRelatedWork W2414846389 @default.
• W2033626471 hasVolume "120" @default.
• W2033626471 isParatext "false" @default.
• W2033626471 isRetracted "false" @default.
• W2033626471 magId "2033626471" @default.
• W2033626471 workType "article" @default.