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• W2033640025 endingPage "3440" @default.
• W2033640025 startingPage "3431" @default.
• W2033640025 abstract "Two-dimensional scaffolds, three-dimensional scaffolds, and dermal substitutes are extensively used for biomedical applications in skin tissue regeneration. Not much explored synthetic polymers, like poly(l-lactic acid)-co-poly-(ε-caprolactone) (PLACL), natural polymers, like silk fibroin (SF), and active inducing agents, such as ascorbic acid (AA) and tetracycline hydrochloride (TCH), represent a favorable matrix for fabricating dermal substitutes to engineer artificial skin for wound repair. The profligate nature of residing skin cells near the wound site is a paramount to survival and also regulating stem cells and other cellular networks and mechanical forces. PLACL/SF/TCH/AA nanofibrous scaffolds were fabricated by electrospinning and characterized for fiber morphology, membrane porosity, wettability, and significant subchains using Fourier transform infrared spectroscopy for culturing human-derived dermal fibroblasts. The PLACL, PLACL/SF, PLACL/SF/TCH, and PLACL/SF/TCH/AA scaffolds obtained diameters between 250 and 340 nm. The secretion of collagen by the laboratory-grown fibroblasts over the AA-blended scaffolds was found to be significantly higher compared with that of other scaffolds. The obtained results positively prove that introduction of naturally secreting compounds (AA) by the cells into the nanofibrous scaffolds will favor cell's microenvironment and eventually leads to complete tissue regeneration. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3431–3440, 2015." @default.
• W2033640025 created "2016-06-24" @default.
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• W2033640025 date "2015-05-06" @default.
• W2033640025 modified "2023-10-18" @default.
• W2033640025 title "Improved regeneration potential of fibroblasts using ascorbic acid-blended nanofibrous scaffolds" @default.
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• W2033640025 doi "https://doi.org/10.1002/jbm.a.35486" @default.
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