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- W2033642402 abstract "We have developed and characterized a new low-cost enzymatic preparation for milk whey saccharification. The preparation consists of β-galactosidase-rich whole cells of the yeast Kluyveromyces lactis, previously cultured on milk whey and immobilized by covalent linkage to corn grits (an inexpensive material). Permeabilization of immobilized cells with ethanol increased the measured values of intracellular β-galactosidase activity up to 240-fold. Permeabilized immobilized cells hydrolyzed milk whey lactose more rapidly than untreated cells, and did not further metabolize the glucose and galactose produced. More than 90% milk whey lactose hydrolysis was achieved in a packed-bed bioreactor at 37°C." @default.
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- W2033642402 date "1994-04-01" @default.
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- W2033642402 title "Kluyveromyces lactis immobilization on corn grits for milk whey lactose hydrolysis" @default.
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- W2033642402 doi "https://doi.org/10.1016/0141-0229(94)90171-6" @default.
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