Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033645911> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2033645911 endingPage "839" @default.
- W2033645911 startingPage "833" @default.
- W2033645911 abstract "A redoxyendonuclease from calf thymus was purified to apparent homogeneity. The redoxyendonuclease recognized and induced cleavage of DNA damaged by ultraviolet light. The enzyme preparation produced a single band of a relative molecular mass of approximately 34 kDa upon SDS/PAGE. The apurinic/apyrimidinic endonuclease and the DNA glycosylase activities remained associated in the apparently homogeneous preparation of the enzyme. The redoxyendonuclease activity displayed a broad pH optimum between pH 5.0-8.5 and exhibited no requirement for divalent cations. By application of FPLC columns Mono-S, Mono-Q and Mono-P, the isoelectric point (pI) of the enzyme was found to be approximately 8.0. Using the DNA sequencing procedure of Maxam and Gilbert [Maxam, A. M. & Gilbert, W. (1980) Methods Enzymol. 65, 499-560] the purified enzyme was found to incise ultraviolet-light-irradiated DNA at pyrimidine sites as observed previously with a more crude form of the enzyme. While the most frequently cleavaged sites for the crude preparation were at cytosine residues, the apparently homogeneous enzyme preparation frequently induced cleavage sites at both cytosine and guanine residues. Predominant incision induced by the apparently homogeneous preparation was observed at guanine residues when a particular DNA sequence was used as substrate. Furthermore, the 16 N-terminal amino acid residues of the purified enzyme were identified. The sequence did not show any significant similarity to other known proteins." @default.
- W2033645911 created "2016-06-24" @default.
- W2033645911 creator A5025020416 @default.
- W2033645911 creator A5059646281 @default.
- W2033645911 creator A5082526389 @default.
- W2033645911 date "1992-06-01" @default.
- W2033645911 modified "2023-10-17" @default.
- W2033645911 title "Purification to homogeneity and characterization of a redoxyendonuclease from calf thymus" @default.
- W2033645911 cites W1558516755 @default.
- W2033645911 cites W1570917001 @default.
- W2033645911 cites W1599617947 @default.
- W2033645911 cites W1786981952 @default.
- W2033645911 cites W1932052686 @default.
- W2033645911 cites W1964484839 @default.
- W2033645911 cites W1985988286 @default.
- W2033645911 cites W1989776390 @default.
- W2033645911 cites W1999791221 @default.
- W2033645911 cites W2009310436 @default.
- W2033645911 cites W2011634180 @default.
- W2033645911 cites W2015292449 @default.
- W2033645911 cites W2024777992 @default.
- W2033645911 cites W2059693060 @default.
- W2033645911 cites W2062957329 @default.
- W2033645911 cites W2072794943 @default.
- W2033645911 cites W2084695608 @default.
- W2033645911 cites W2088683419 @default.
- W2033645911 cites W2093026457 @default.
- W2033645911 cites W2100837269 @default.
- W2033645911 cites W2121629473 @default.
- W2033645911 doi "https://doi.org/10.1111/j.1432-1033.1992.tb16991.x" @default.
- W2033645911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1376689" @default.
- W2033645911 hasPublicationYear "1992" @default.
- W2033645911 type Work @default.
- W2033645911 sameAs 2033645911 @default.
- W2033645911 citedByCount "4" @default.
- W2033645911 crossrefType "journal-article" @default.
- W2033645911 hasAuthorship W2033645911A5025020416 @default.
- W2033645911 hasAuthorship W2033645911A5059646281 @default.
- W2033645911 hasAuthorship W2033645911A5082526389 @default.
- W2033645911 hasBestOaLocation W20336459111 @default.
- W2033645911 hasConcept C104317684 @default.
- W2033645911 hasConcept C143425029 @default.
- W2033645911 hasConcept C150777479 @default.
- W2033645911 hasConcept C153911025 @default.
- W2033645911 hasConcept C181199279 @default.
- W2033645911 hasConcept C185592680 @default.
- W2033645911 hasConcept C192396546 @default.
- W2033645911 hasConcept C2776023528 @default.
- W2033645911 hasConcept C2777028655 @default.
- W2033645911 hasConcept C2778301229 @default.
- W2033645911 hasConcept C2987116853 @default.
- W2033645911 hasConcept C50476208 @default.
- W2033645911 hasConcept C512185932 @default.
- W2033645911 hasConcept C552990157 @default.
- W2033645911 hasConcept C55493867 @default.
- W2033645911 hasConcept C75473681 @default.
- W2033645911 hasConcept C86803240 @default.
- W2033645911 hasConcept C96294017 @default.
- W2033645911 hasConceptScore W2033645911C104317684 @default.
- W2033645911 hasConceptScore W2033645911C143425029 @default.
- W2033645911 hasConceptScore W2033645911C150777479 @default.
- W2033645911 hasConceptScore W2033645911C153911025 @default.
- W2033645911 hasConceptScore W2033645911C181199279 @default.
- W2033645911 hasConceptScore W2033645911C185592680 @default.
- W2033645911 hasConceptScore W2033645911C192396546 @default.
- W2033645911 hasConceptScore W2033645911C2776023528 @default.
- W2033645911 hasConceptScore W2033645911C2777028655 @default.
- W2033645911 hasConceptScore W2033645911C2778301229 @default.
- W2033645911 hasConceptScore W2033645911C2987116853 @default.
- W2033645911 hasConceptScore W2033645911C50476208 @default.
- W2033645911 hasConceptScore W2033645911C512185932 @default.
- W2033645911 hasConceptScore W2033645911C552990157 @default.
- W2033645911 hasConceptScore W2033645911C55493867 @default.
- W2033645911 hasConceptScore W2033645911C75473681 @default.
- W2033645911 hasConceptScore W2033645911C86803240 @default.
- W2033645911 hasConceptScore W2033645911C96294017 @default.
- W2033645911 hasIssue "3" @default.
- W2033645911 hasLocation W20336459111 @default.
- W2033645911 hasLocation W20336459112 @default.
- W2033645911 hasOpenAccess W2033645911 @default.
- W2033645911 hasPrimaryLocation W20336459111 @default.
- W2033645911 hasRelatedWork W2031101499 @default.
- W2033645911 hasRelatedWork W2063163055 @default.
- W2033645911 hasRelatedWork W2068220611 @default.
- W2033645911 hasRelatedWork W2144712334 @default.
- W2033645911 hasRelatedWork W2168882862 @default.
- W2033645911 hasRelatedWork W2528508621 @default.
- W2033645911 hasRelatedWork W4231593990 @default.
- W2033645911 hasRelatedWork W4378835358 @default.
- W2033645911 hasRelatedWork W4380992534 @default.
- W2033645911 hasRelatedWork W53635053 @default.
- W2033645911 hasVolume "206" @default.
- W2033645911 isParatext "false" @default.
- W2033645911 isRetracted "false" @default.
- W2033645911 magId "2033645911" @default.
- W2033645911 workType "article" @default.