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- W2033704508 abstract "In order to study the influence of temperature on vitellogenin gene and estrogen receptor gene expression in primary hepatocytes from rainbow trout (Oncorhynchus mykiss), cells were exposed to 17β-estradiol, bisphenol-A and nonylphenol for 48 and 96 hr. Induction of vitellogenin-mRNA expression was detected in a non-radioactive dot blot/RNAse protection assay and by RT-PCR. In the dot blot/RNAse protection assay, the estrogenic potentials of bisphenol-A and nonylphenol were about 104- to 105-fold and 105-fold lower than that of 17β-estradiol, respectively. The relative estrogenic potential did not show any difference between 14 and 18°C. In contrast, at 18°C, RT-PCR analysis revealed increased amounts of vitellogenin- and estrogen receptor-mRNA after 12 and 24 hr of exposure to 17β-estradiol, if compared to 14°C. Owing to increased vitellogenin gene expression at 18°C, the sensitivity of primary hepatocytes to 17β-estradiol and bisphenol-A could be increased." @default.
- W2033704508 created "2016-06-24" @default.
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- W2033704508 date "2000-12-01" @default.
- W2033704508 modified "2023-10-18" @default.
- W2033704508 title "Temperature-dependent vitellogenin-mRNA expression in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes at 14 and 18°C" @default.
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- W2033704508 doi "https://doi.org/10.1016/s0887-2333(00)00048-5" @default.
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