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- W2033723786 abstract "Abstract HeLa cells were exposed to 32P for 10, 30 and 60 min in Eagle's medium containing reduced amounts of phosphate. The nuclei of these cells were isolated by a modified citric acid method and the relative base composition of the labelled RNA of the nuclei determined after each time interval. In another set of experiments, the HeLa cells were exposed to 32P for the same time intervals, the radioactive medium was removed and the cells were incubated in normal Eagle's medium for 8 h to permit the migration of the labelled RNA from the nucleus to the cytoplasm. At the end of this period, the nuclei were isolated and the relative base composition was determined. A comparison of the base compositions of the labelled RNA of the nuclei “before and after migration” indicated that the migratory species of RNA is rich in adenylic and uridylic acids and poor in guanylic and cytidylic acids at the three time intervals investigated. These observations are discussed in the light of our present understanding of the different types of RNA found in cells." @default.
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- W2033723786 date "1962-10-01" @default.
- W2033723786 modified "2023-09-22" @default.
- W2033723786 title "On the nature of the ribonucleic acids migrating from the nucleus to the cytoplasm in HeLa cells" @default.
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- W2033723786 doi "https://doi.org/10.1016/0926-6550(62)90103-2" @default.
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