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• W2033725438 abstract "A sensitive enantioselective high performance liquid chromatographic assay for determination of the dihydropyridine-type calcium antagonist amlodipine in human plasma samples is described. Chiral chromatography is performed on an α1-acid glycoprotein column (i.e. Chiral-AGP) and the eluted enantiomers are trapped and compressed on two short columns before final achiral chromatography on a narrow bore column (i.e. Zorbax SB-Ph) using electrochemical detection. Both stereoselective quantitative analysis and enantiomeric ratio analysis, for samples with a known total concentration of amlodipine are described. The quantitative assay shows linearity over the range 0.5–10 ng ml−1 for the two enantiomers and the limit of detection is about 0.2 ng ml−1. The method has been applied to a pharmacokinetic study of the enantiomers of amlodipine in human subjects." @default.
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• W2033725438 date "1996-11-01" @default.
• W2033725438 modified "2023-09-27" @default.
• W2033725438 title "Coupled-column chromatography on a Chiral-AGP phase for determination of amlodipine enantiomers in human plasma: An HPLC assay with electrochemical detection" @default.
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• W2033725438 doi "https://doi.org/10.1016/0731-7085(96)01836-5" @default.
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