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• W2033728137 abstract "The aim of this project was to establish a method for the purification of total-RNA from fixed rat-retina. Two different established methods were used for RNA purification, and successful isolation was verified with RT-PCR for amplification of β-actin (two different product-lengths) and subsequent gel-electrophoresis. Total-RNA was successfully isolated from fixed rat-retina. The house keeping gene, β-actin could be detected after fixing the retina either with 1% formalin or with 4% paraformaldehyde (PFA). Hexamer-primer based RT-PCR gave better results than the oligo-d(T)-primer based RT-PCR method. Both the 698 and 225 bp β-actin-fragments could be successfully amplified, where amplification of the latter was more efficient. This approach shows that tissue fixation prior to RNA-isolation facilitates the rapid isolation of undamaged RNAs in tissues such as the retina, which are known to yield low levels of RNA and are vulnerable to RNases." @default.
• W2033728137 created "2016-06-24" @default.
• W2033728137 creator A5003030738 @default.
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• W2033728137 date "2002-10-01" @default.
• W2033728137 modified "2023-09-26" @default.
• W2033728137 title "Isolation of total-RNA from formalin-fixed rat retina" @default.
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• W2033728137 doi "https://doi.org/10.1016/s0165-0270(02)00188-7" @default.
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