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- W2033747992 abstract "Angiostrongylus vasorum is an emerging parasite of dogs and related carnivores throughout western Europe and presents a biosecurity threat to many countries worldwide. Infections are difficult to diagnose due to a high variability of clinical signs and can be fatal if left untreated. Previous attempts to develop indirect ELISA as a diagnostic test for antibody presence in dogs have been limited by cross-reactive recognition of antibodies to other endemic nematodes. This study compared the immuno-dominant soluble somatic proteins for first stage, third stage and adult A. vasorum using Western blotting. 2D electrophoresis and mass spectrometry were further used to identify specific immuno-dominant proteins (n=14) within the soluble fraction from whole adult worms. Proteins included an aspartyl protease inhibitor, representing a potential candidate for a recombinant-based ELISA and novel vaccine." @default.
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- W2033747992 date "2011-11-01" @default.
- W2033747992 modified "2023-09-24" @default.
- W2033747992 title "Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry" @default.
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- W2033747992 doi "https://doi.org/10.1016/j.molbiopara.2011.07.009" @default.
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