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- W2033751566 abstract "The optimization of the separation of recombinant human insulin (rhI) and recombinant human proinsulin (rhP) by free-solution capillary zone electrophoresis in uncoated fused-silica capillaries with ionic and zwitterionic buffers was carried out. The relationship between the selectivity and pH of the buffer was established. The effects of pH and ionic strength of the buffer on protein adsorption on the capillary walls and Taylor diffusion was investigated. The separation of rhI and rhP with an efficiency of 200 000 theoretical plates was achieved using 20 m M Na 2 HPO 4 NaOH buffer (pH 11.2). The proposed method allows the simultaneously determination of rhP and some rhI degradation products (which are also included in pharmacopoeias) in pharmacopoeially limited amounts in rhI." @default.
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- W2033751566 date "1994-12-01" @default.
- W2033751566 modified "2023-09-27" @default.
- W2033751566 title "Recombinant human insulin VI. Determination of recombinant human proinsulin by capillary zone electrophoresis: optimization of efficiency and selectivity" @default.
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- W2033751566 doi "https://doi.org/10.1016/0378-4347(94)00219-3" @default.
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