FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033792606> ?p ?o ?g. }

• W2033792606 endingPage "2932" @default.
• W2033792606 startingPage "2919" @default.
• W2033792606 abstract "A procedure for the construction of general cDNA libraries is described which is based on the amplification of total cDNA in vitro. The first cDNA strand is synthesized from total RNA using an oligo(dT)-containing primer. After oligo(dG) tailing the total cDNA is amplified by PCR using two primers complementary to oligo(dA) and oligo(dG) ends of the cDNA. For insertion of the cDNA into a vector a controlled trimming of the 3' ends of the cDNA by Klenow enzyme was used. Starting from 10 J558L micron3 myeloma cells, total cDNA was synthesized and amplified approximately 10(5) fold. A library containing 10(6) clones was established from 1/6 of the amplified cDNA. Screening of the library with probes for three genes expressed in these cells revealed a number of corresponding clones in each case. The longest obtained clones contained inserts of 1.5 kb length. No sequences originating from carriers or from rRNA was found in 14 randomly picked clones." @default.
• W2033792606 created "2016-06-24" @default.
• W2033792606 creator A5012958604 @default.
• W2033792606 creator A5020790185 @default.
• W2033792606 creator A5028599195 @default.
• W2033792606 date "1989-01-01" @default.
• W2033792606 modified "2023-10-16" @default.
• W2033792606 title "PCR-based cDNA library construction: general cDNA libraries at the level of a few cells" @default.
• W2033792606 cites W1007536749 @default.
• W2033792606 cites W1488727016 @default.
• W2033792606 cites W1510175790 @default.
• W2033792606 cites W1977833662 @default.
• W2033792606 cites W1993008017 @default.
• W2033792606 cites W2021673452 @default.
• W2033792606 cites W2024988062 @default.
• W2033792606 cites W2032118018 @default.
• W2033792606 cites W2032462421 @default.
• W2033792606 cites W2034168176 @default.
• W2033792606 cites W2035751667 @default.
• W2033792606 cites W2050717506 @default.
• W2033792606 cites W2051112456 @default.
• W2033792606 cites W2060333964 @default.
• W2033792606 cites W2064421628 @default.
• W2033792606 cites W2085062936 @default.
• W2033792606 cites W2087995258 @default.
• W2033792606 cites W2092784580 @default.
• W2033792606 cites W2093617423 @default.
• W2033792606 cites W2108388593 @default.
• W2033792606 cites W2134812217 @default.
• W2033792606 cites W2282468893 @default.
• W2033792606 cites W240934071 @default.
• W2033792606 cites W2409738534 @default.
• W2033792606 cites W2576734941 @default.
• W2033792606 cites W36295273 @default.
• W2033792606 cites W88134075 @default.
• W2033792606 cites W2764264365 @default.
• W2033792606 doi "https://doi.org/10.1093/nar/17.8.2919" @default.
• W2033792606 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/317702" @default.
• W2033792606 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2471144" @default.
• W2033792606 hasPublicationYear "1989" @default.
• W2033792606 type Work @default.
• W2033792606 sameAs 2033792606 @default.
• W2033792606 citedByCount "280" @default.
• W2033792606 countsByYear W20337926062012 @default.
• W2033792606 countsByYear W20337926062014 @default.
• W2033792606 countsByYear W20337926062015 @default.
• W2033792606 countsByYear W20337926062016 @default.
• W2033792606 countsByYear W20337926062017 @default.
• W2033792606 countsByYear W20337926062019 @default.
• W2033792606 countsByYear W20337926062020 @default.
• W2033792606 countsByYear W20337926062021 @default.
• W2033792606 countsByYear W20337926062023 @default.
• W2033792606 crossrefType "journal-article" @default.
• W2033792606 hasAuthorship W2033792606A5012958604 @default.
• W2033792606 hasAuthorship W2033792606A5020790185 @default.
• W2033792606 hasAuthorship W2033792606A5028599195 @default.
• W2033792606 hasBestOaLocation W20337926062 @default.
• W2033792606 hasConcept C104317684 @default.
• W2033792606 hasConcept C127413603 @default.
• W2033792606 hasConcept C1436000 @default.
• W2033792606 hasConcept C153911025 @default.
• W2033792606 hasConcept C178790620 @default.
• W2033792606 hasConcept C185592680 @default.
• W2033792606 hasConcept C187882448 @default.
• W2033792606 hasConcept C189482833 @default.
• W2033792606 hasConcept C19924922 @default.
• W2033792606 hasConcept C2777563447 @default.
• W2033792606 hasConcept C2780098074 @default.
• W2033792606 hasConcept C42062724 @default.
• W2033792606 hasConcept C50724178 @default.
• W2033792606 hasConcept C54355233 @default.
• W2033792606 hasConcept C78519656 @default.
• W2033792606 hasConcept C86803240 @default.
• W2033792606 hasConceptScore W2033792606C104317684 @default.
• W2033792606 hasConceptScore W2033792606C127413603 @default.
• W2033792606 hasConceptScore W2033792606C1436000 @default.
• W2033792606 hasConceptScore W2033792606C153911025 @default.
• W2033792606 hasConceptScore W2033792606C178790620 @default.
• W2033792606 hasConceptScore W2033792606C185592680 @default.
• W2033792606 hasConceptScore W2033792606C187882448 @default.
• W2033792606 hasConceptScore W2033792606C189482833 @default.
• W2033792606 hasConceptScore W2033792606C19924922 @default.
• W2033792606 hasConceptScore W2033792606C2777563447 @default.
• W2033792606 hasConceptScore W2033792606C2780098074 @default.
• W2033792606 hasConceptScore W2033792606C42062724 @default.
• W2033792606 hasConceptScore W2033792606C50724178 @default.
• W2033792606 hasConceptScore W2033792606C54355233 @default.
• W2033792606 hasConceptScore W2033792606C78519656 @default.
• W2033792606 hasConceptScore W2033792606C86803240 @default.
• W2033792606 hasIssue "8" @default.
• W2033792606 hasLocation W20337926061 @default.
• W2033792606 hasLocation W20337926062 @default.
• W2033792606 hasLocation W20337926063 @default.
• W2033792606 hasLocation W20337926064 @default.
• W2033792606 hasOpenAccess W2033792606 @default.
• W2033792606 hasPrimaryLocation W20337926061 @default.
• W2033792606 hasRelatedWork W1730115118 @default.
• W2033792606 hasRelatedWork W1973254433 @default.

• next