FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033804950> ?p ?o ?g. }

• W2033804950 endingPage "743" @default.
• W2033804950 startingPage "739" @default.
• W2033804950 abstract "SIRT1, human ortholog of yeast SIR2 protein, deacetylates histones and several other transcription factors. Recently, SIRT1 has emerged as a drug target for treating age related diseases, type II diabetes, neurodegeneration, inflammation and cancer. Here, we have optimized production of functionally active wild type full-length SIRT1 protein and its N-terminal deleted mutants. In a comparative study, we found that the region containing 192–208 amino acids towards the N-terminus is critical for right conformational folding of the protein to retain its deacetylase activity. The EC50 and IC50 values obtained with standard modulators showed that the SRT748 & SRT556 can deacetylate substrate and are activated by resveratrol, whereas, deacetylase activity of all the other deletion mutants (SRT540, SRT532, SRT507 and SRT503) was lost. We further report that the peptide substrate Km for SRT748 (70 ± 5.2 μM) was comparable to SRT556 (93 ± 5.4 μM). The Km for NAD+ substrate was 176 & 274 μM for SRT748 and SRT556, respectively. Similar substrate affinity studies demonstrate that either of the protein (SRT748 or SRT556) can be utilized for screening SIRT1 modulators. We have also examined critical regions in SIRT1 required for deacetylase activity as well as kinetic analyses of SIRT1 proteins." @default.
• W2033804950 created "2016-06-24" @default.
• W2033804950 creator A5000430352 @default.
• W2033804950 creator A5007218289 @default.
• W2033804950 creator A5031179904 @default.
• W2033804950 creator A5034128325 @default.
• W2033804950 creator A5044432767 @default.
• W2033804950 creator A5047185413 @default.
• W2033804950 creator A5065207233 @default.
• W2033804950 creator A5076965750 @default.
• W2033804950 creator A5082950754 @default.
• W2033804950 date "2010-01-01" @default.
• W2033804950 modified "2023-09-27" @default.
• W2033804950 title "Comparative deacetylase activity of wild type and mutants of SIRT1" @default.
• W2033804950 cites W1969408132 @default.
• W2033804950 cites W1985308585 @default.
• W2033804950 cites W1987178054 @default.
• W2033804950 cites W1996094302 @default.
• W2033804950 cites W1996395636 @default.
• W2033804950 cites W2001346869 @default.
• W2033804950 cites W2018587074 @default.
• W2033804950 cites W2039613373 @default.
• W2033804950 cites W2044578262 @default.
• W2033804950 cites W2052815426 @default.
• W2033804950 cites W2054829361 @default.
• W2033804950 cites W2060814375 @default.
• W2033804950 cites W2076743693 @default.
• W2033804950 cites W2088381487 @default.
• W2033804950 cites W2107733280 @default.
• W2033804950 cites W2116350182 @default.
• W2033804950 cites W2121431024 @default.
• W2033804950 cites W2124595934 @default.
• W2033804950 cites W2124898423 @default.
• W2033804950 cites W2130503166 @default.
• W2033804950 cites W2140925956 @default.
• W2033804950 cites W2157649587 @default.
• W2033804950 cites W2158153532 @default.
• W2033804950 cites W2161532608 @default.
• W2033804950 doi "https://doi.org/10.1016/j.bbrc.2009.11.130" @default.
• W2033804950 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19944070" @default.
• W2033804950 hasPublicationYear "2010" @default.
• W2033804950 type Work @default.
• W2033804950 sameAs 2033804950 @default.
• W2033804950 citedByCount "6" @default.
• W2033804950 countsByYear W20338049502012 @default.
• W2033804950 countsByYear W20338049502014 @default.
• W2033804950 crossrefType "journal-article" @default.
• W2033804950 hasAuthorship W2033804950A5000430352 @default.
• W2033804950 hasAuthorship W2033804950A5007218289 @default.
• W2033804950 hasAuthorship W2033804950A5031179904 @default.
• W2033804950 hasAuthorship W2033804950A5034128325 @default.
• W2033804950 hasAuthorship W2033804950A5044432767 @default.
• W2033804950 hasAuthorship W2033804950A5047185413 @default.
• W2033804950 hasAuthorship W2033804950A5065207233 @default.
• W2033804950 hasAuthorship W2033804950A5076965750 @default.
• W2033804950 hasAuthorship W2033804950A5082950754 @default.
• W2033804950 hasBestOaLocation W20338049501 @default.
• W2033804950 hasConcept C104317684 @default.
• W2033804950 hasConcept C143065580 @default.
• W2033804950 hasConcept C153911025 @default.
• W2033804950 hasConcept C181199279 @default.
• W2033804950 hasConcept C185592680 @default.
• W2033804950 hasConcept C207583985 @default.
• W2033804950 hasConcept C2778305200 @default.
• W2033804950 hasConcept C55493867 @default.
• W2033804950 hasConcept C64927066 @default.
• W2033804950 hasConcept C75520062 @default.
• W2033804950 hasConcept C86803240 @default.
• W2033804950 hasConceptScore W2033804950C104317684 @default.
• W2033804950 hasConceptScore W2033804950C143065580 @default.
• W2033804950 hasConceptScore W2033804950C153911025 @default.
• W2033804950 hasConceptScore W2033804950C181199279 @default.
• W2033804950 hasConceptScore W2033804950C185592680 @default.
• W2033804950 hasConceptScore W2033804950C207583985 @default.
• W2033804950 hasConceptScore W2033804950C2778305200 @default.
• W2033804950 hasConceptScore W2033804950C55493867 @default.
• W2033804950 hasConceptScore W2033804950C64927066 @default.
• W2033804950 hasConceptScore W2033804950C75520062 @default.
• W2033804950 hasConceptScore W2033804950C86803240 @default.
• W2033804950 hasIssue "1" @default.
• W2033804950 hasLocation W20338049501 @default.
• W2033804950 hasLocation W20338049502 @default.
• W2033804950 hasOpenAccess W2033804950 @default.
• W2033804950 hasPrimaryLocation W20338049501 @default.
• W2033804950 hasRelatedWork W1508691356 @default.
• W2033804950 hasRelatedWork W1931404067 @default.
• W2033804950 hasRelatedWork W2009464217 @default.
• W2033804950 hasRelatedWork W2049887237 @default.
• W2033804950 hasRelatedWork W2074191566 @default.
• W2033804950 hasRelatedWork W2086538185 @default.
• W2033804950 hasRelatedWork W2314620439 @default.
• W2033804950 hasRelatedWork W2520746134 @default.
• W2033804950 hasRelatedWork W4284988915 @default.
• W2033804950 hasRelatedWork W214510971 @default.
• W2033804950 hasVolume "391" @default.
• W2033804950 isParatext "false" @default.
• W2033804950 isRetracted "false" @default.
• W2033804950 magId "2033804950" @default.

• next