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- W2033883874 abstract "The outer membrane proteins YadA and invasin of Yersinia pseudotuberculosis promote invasion into mammalian cells through β1-integrins and trigger the production of interleukin (IL)-8. FAK, c-Src and the PI3 kinase were previously found to be important for both YadA- and invasin-promoted uptake. Here, we demonstrate that two different downstream effectors of PI3 kinase, Akt and phospholipase Cγ1 are required for efficient cell invasion. Inhibition of Akt or phospholipase C-γ (PLC-γ)1 by pharmaceutical agents as well as reduced expression of the isoforms Akt1 and Akt2, and of PLC-γ1 by RNA interference decreased entry of YadA- and Inv-expressing bacteria significantly. In addition, we report that the conventional protein kinases C (PKC)α and -β, positioned downstream of PLC-γ1, are activated upon Inv- or YadA-promoted cell entry. They colocalize with intracellular bacteria and their depletion by siRNA treatment also resulted in a strong reduction of cell entry. In contrast, neither Akt nor PLC-γ1, and the PKCs are essential for YadA- and Inv-mediated IL-8 synthesis and release. We conclude that YadA and invasin of Y. pseudotuberculosis both trigger similar signal transduction pathways during integrin-mediated phagocytosis into epithelial cells, which lead to the activation of Akt, PLC-γ1, PKCα and -β downstream of PI3 kinase, separate from the MAPK-dependent pathway that triggers IL-8 production." @default.
- W2033883874 created "2016-06-24" @default.
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- W2033883874 creator A5085712721 @default.
- W2033883874 date "2009-08-13" @default.
- W2033883874 modified "2023-10-16" @default.
- W2033883874 title "Cell invasion of<i>Yersinia pseudotuberculosis</i>by invasin and YadA requires protein kinase C, phospholipase C-γ1 and Akt kinase" @default.
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- W2033883874 doi "https://doi.org/10.1111/j.1462-5822.2009.01371.x" @default.
- W2033883874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19681907" @default.
- W2033883874 hasPublicationYear "2009" @default.
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