Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033884043> ?p ?o ?g. }
- W2033884043 endingPage "981" @default.
- W2033884043 startingPage "973" @default.
- W2033884043 abstract "Aims: To devise and evaluate a strategy for isolating members of the Streptomyces violaceusniger phenotypic cluster, which are known to be a promising source of bioactive metabolites. Methods and Results: The treatment of four soil samples with 1·5% phenol (30°C, 30 min) prior to inoculation on humic acid‐vitamin (HV) agar eliminated most of the streptomycetes and other bacterial populations. The surviving streptomycetes on the HV isolation plates were subcultured, and species‐group identification was made according to the probabilistic identification system of Williams et al. (1989). Of the 133 streptomycetes subcultured, 102 (77%), were assigned to the S. violaceusniger cluster. A test with an overlay technique revealed that all of these S. violaceusniger‐cluster isolates had broad antimicrobial spectra, as they inhibited the growth of all test Gram‐positive bacteria, yeasts and filamentous fungi. Antitumour activity against colon carcinoma cells was found among 68 or 67%, of these S. violaceusniger‐cluster isolates, following growth in submerged culture. Conclusions: Chemical pretreatment of soil samples with phenol reduces the growth of ubiquitous Streptomyces species, thereby facilitating the recovery of less‐abundant S. violaceusniger‐cluster strains that are characterized by high antimicrobial and antitumour activities. Significance and Impact of the Study: The development and application of new methodologies with which to selectively isolate rare, bioactive streptomycete groups is important for discovering novel secondary metabolites with bioactive properties." @default.
- W2033884043 created "2016-06-24" @default.
- W2033884043 creator A5021341455 @default.
- W2033884043 creator A5021758173 @default.
- W2033884043 creator A5033272579 @default.
- W2033884043 date "2004-05-01" @default.
- W2033884043 modified "2023-10-06" @default.
- W2033884043 title "Selective isolation of bioactive soil actinomycetes belonging to the Streptomyces violaceusniger phenotypic cluster" @default.
- W2033884043 cites W1534400849 @default.
- W2033884043 cites W1594979181 @default.
- W2033884043 cites W1672200396 @default.
- W2033884043 cites W1822642935 @default.
- W2033884043 cites W1966446157 @default.
- W2033884043 cites W1968129689 @default.
- W2033884043 cites W1981053691 @default.
- W2033884043 cites W2003554089 @default.
- W2033884043 cites W2009491770 @default.
- W2033884043 cites W2013003830 @default.
- W2033884043 cites W2018244833 @default.
- W2033884043 cites W2042554314 @default.
- W2033884043 cites W2043414578 @default.
- W2033884043 cites W2044536177 @default.
- W2033884043 cites W2046604694 @default.
- W2033884043 cites W2051545115 @default.
- W2033884043 cites W2053531240 @default.
- W2033884043 cites W2060443822 @default.
- W2033884043 cites W2061033766 @default.
- W2033884043 cites W2066131449 @default.
- W2033884043 cites W2067723204 @default.
- W2033884043 cites W2085771892 @default.
- W2033884043 cites W2095527895 @default.
- W2033884043 cites W2102049461 @default.
- W2033884043 cites W2124233578 @default.
- W2033884043 cites W2127122957 @default.
- W2033884043 cites W2154171815 @default.
- W2033884043 cites W2157521882 @default.
- W2033884043 cites W2158837052 @default.
- W2033884043 cites W2285949578 @default.
- W2033884043 cites W2316370121 @default.
- W2033884043 cites W2396699816 @default.
- W2033884043 cites W2401856577 @default.
- W2033884043 cites W2484939131 @default.
- W2033884043 cites W2486926789 @default.
- W2033884043 cites W2504856998 @default.
- W2033884043 cites W2517054293 @default.
- W2033884043 cites W3025546593 @default.
- W2033884043 cites W4236247425 @default.
- W2033884043 cites W4248497453 @default.
- W2033884043 cites W4292963792 @default.
- W2033884043 doi "https://doi.org/10.1111/j.1365-2672.2004.02230.x" @default.
- W2033884043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15078513" @default.
- W2033884043 hasPublicationYear "2004" @default.
- W2033884043 type Work @default.
- W2033884043 sameAs 2033884043 @default.
- W2033884043 citedByCount "76" @default.
- W2033884043 countsByYear W20338840432012 @default.
- W2033884043 countsByYear W20338840432013 @default.
- W2033884043 countsByYear W20338840432014 @default.
- W2033884043 countsByYear W20338840432015 @default.
- W2033884043 countsByYear W20338840432016 @default.
- W2033884043 countsByYear W20338840432017 @default.
- W2033884043 countsByYear W20338840432018 @default.
- W2033884043 countsByYear W20338840432019 @default.
- W2033884043 countsByYear W20338840432020 @default.
- W2033884043 countsByYear W20338840432021 @default.
- W2033884043 countsByYear W20338840432022 @default.
- W2033884043 countsByYear W20338840432023 @default.
- W2033884043 crossrefType "journal-article" @default.
- W2033884043 hasAuthorship W2033884043A5021341455 @default.
- W2033884043 hasAuthorship W2033884043A5021758173 @default.
- W2033884043 hasAuthorship W2033884043A5033272579 @default.
- W2033884043 hasConcept C2775941552 @default.
- W2033884043 hasConcept C2778660310 @default.
- W2033884043 hasConcept C2779920670 @default.
- W2033884043 hasConcept C31903555 @default.
- W2033884043 hasConcept C4937899 @default.
- W2033884043 hasConcept C523546767 @default.
- W2033884043 hasConcept C54355233 @default.
- W2033884043 hasConcept C86803240 @default.
- W2033884043 hasConcept C89423630 @default.
- W2033884043 hasConceptScore W2033884043C2775941552 @default.
- W2033884043 hasConceptScore W2033884043C2778660310 @default.
- W2033884043 hasConceptScore W2033884043C2779920670 @default.
- W2033884043 hasConceptScore W2033884043C31903555 @default.
- W2033884043 hasConceptScore W2033884043C4937899 @default.
- W2033884043 hasConceptScore W2033884043C523546767 @default.
- W2033884043 hasConceptScore W2033884043C54355233 @default.
- W2033884043 hasConceptScore W2033884043C86803240 @default.
- W2033884043 hasConceptScore W2033884043C89423630 @default.
- W2033884043 hasIssue "5" @default.
- W2033884043 hasLocation W20338840431 @default.
- W2033884043 hasLocation W20338840432 @default.
- W2033884043 hasOpenAccess W2033884043 @default.
- W2033884043 hasPrimaryLocation W20338840431 @default.
- W2033884043 hasRelatedWork W1601372800 @default.
- W2033884043 hasRelatedWork W2035337314 @default.
- W2033884043 hasRelatedWork W2070232054 @default.
- W2033884043 hasRelatedWork W2098006894 @default.