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• W2033963721 endingPage "127" @default.
• W2033963721 startingPage "121" @default.
• W2033963721 abstract "The α-I domain, found in the α-subunit of the leucocyte integrins such as αMβ2 and αLβ2, switches between the open and closed tertiary conformations, reflecting the high- and low-affinity ligand-binding states of the integrin that are required for regulated cell adhesion and migration. In the present study we show, by using point mutations and engineered disulphide bonds, that ligand affinity can be reduced or increased allosterically by altering the equilibrium between the closed and open states. We determined equilibrium constants for the binding of two ligands, fibrinogen and intercellular cell-adhesion molecule 1, to the αM-I domain by surface plasmon resonance, and determined crystal structures of a low-affinity mutant. Locking the domain in the open conformation increases affinity by a factor of no greater than 10, consistent with a closely balanced equilibrium between the two conformations in the absence of ligand. This behaviour contrasts with that of the unliganded αL-I domain, for which the equilibrium lies strongly in favour of the closed conformation. These results suggest significant differences in the way the parent integrins regulate I domain conformation and hence ligand affinity." @default.
• W2033963721 created "2016-06-24" @default.
• W2033963721 creator A5007919640 @default.
• W2033963721 creator A5068379995 @default.
• W2033963721 date "2003-05-15" @default.
• W2033963721 modified "2023-10-04" @default.
• W2033963721 title "Engineered allosteric mutants of the integrin alphaMbeta2 I domain: structural and functional studies" @default.
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• W2033963721 doi "https://doi.org/10.1042/bj20021273" @default.
• W2033963721 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223386" @default.
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• W2033963721 hasPublicationYear "2003" @default.
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