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- W2033988337 abstract "Distinctive generation of biochemical response patterns of eight different substances, using an assay based on pigment containing cells, was demonstrated. Xenopus laevis melanophores, transfected with human β2-adrenergic receptor, were seeded in a 96 well microplate and used to generate individual biochemical images through a two transient measuring protocol that contributes to highlight the response signatures of the agents. Adequate signal processing creates distinctive patterns in a time–concentration response space suitable for substance classification. The concept of biochemical images is introduced here. The assays were evaluated both with a standard microplate reader and with a computer screen photo-assisted technique (CSPT) yielding similar results. Since CSPT platforms only demand standard computer sets and web cameras as measuring setup, applications for these kind of assays outside main-laboratories were discussed." @default.
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- W2033988337 date "2005-11-01" @default.
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- W2033988337 title "Generation of biochemical response patterns of different substances using a whole cell assay with multiple signaling pathways" @default.
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- W2033988337 doi "https://doi.org/10.1016/j.bios.2005.01.017" @default.
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