FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2033989457> ?p ?o ?g. }

• W2033989457 endingPage "5120" @default.
• W2033989457 startingPage "5113" @default.
• W2033989457 abstract "Abstract The smart surface created in a microfluidic chip has shown the capability of adsorbing and releasing proteins under electrical control. The inner surface of the chip channel was first coated by a thin layer of Au through sputtering and was subsequently modified with loosely packed self‐assembled monolayers (SAMs) of thiols with terminal carboxylic or amino groups. Upon application of an external electric potential to the gold substrate, reversible conformational transformation between “bent” and “straight” states for the anchored mercapto chains could be modulated, through the electrostatic effect between the ionized terminal groups and the charged gold substrate. Thus, a hydrophobic or hydrophilic channel surface was established and could be reversibly switched electrochemically. Accordingly, the microchips prepared in this way can reversibly and selectively adsorb and release differently charged proteins under electrical control. Two model proteins, avidin and streptavidin, were demonstrated to be readily adsorbed by the smart chips under negative and positive potential, respectively. Also, more than 90 % of the adsorbed proteins could be released upon an electrical command. Furthermore, these chips were applied to the controlled separation of avidin and streptavidin mixtures with 1:1 and 1:1000 molar ratios. Under specific applied potentials, the chips adsorbed a certain protein from the mixture whereas the other protein was allowed to flow out, after which the adsorbed protein could be released by switching the applied potential. Thus, two eluted protein fractions were obtained and the separation of the two proteins was achieved. For the former mixture, each eluted fraction contained up to ≈80–90 % avidin or streptavidin. For the latter mixture, the resulting separation efficiency indicated that the molar ratio of avidin and streptavidin could be increased from 1:1000 to about 32:1 after five run separations." @default.
• W2033989457 created "2016-06-24" @default.
• W2033989457 creator A5012535610 @default.
• W2033989457 creator A5051065845 @default.
• W2033989457 creator A5060002817 @default.
• W2033989457 creator A5081325802 @default.
• W2033989457 date "2007-06-01" @default.
• W2033989457 modified "2023-10-17" @default.
• W2033989457 title "A Smart Surface in a Microfluidic Chip for Controlled Protein Separation" @default.
• W2033989457 cites W1512509057 @default.
• W2033989457 cites W1666890712 @default.
• W2033989457 cites W1965447629 @default.
• W2033989457 cites W1966751543 @default.
• W2033989457 cites W1967022451 @default.
• W2033989457 cites W1970226704 @default.
• W2033989457 cites W1974743608 @default.
• W2033989457 cites W1975130453 @default.
• W2033989457 cites W1982366874 @default.
• W2033989457 cites W1983642598 @default.
• W2033989457 cites W1986643982 @default.
• W2033989457 cites W1990913686 @default.
• W2033989457 cites W1991977252 @default.
• W2033989457 cites W2002997951 @default.
• W2033989457 cites W2008046073 @default.
• W2033989457 cites W2012418542 @default.
• W2033989457 cites W2013117405 @default.
• W2033989457 cites W2016831453 @default.
• W2033989457 cites W2020328498 @default.
• W2033989457 cites W2022520585 @default.
• W2033989457 cites W2029921815 @default.
• W2033989457 cites W2040341666 @default.
• W2033989457 cites W2042386191 @default.
• W2033989457 cites W2079535935 @default.
• W2033989457 cites W2085621045 @default.
• W2033989457 cites W2086525212 @default.
• W2033989457 cites W2087370260 @default.
• W2033989457 cites W2088101853 @default.
• W2033989457 cites W2089622237 @default.
• W2033989457 cites W2091082294 @default.
• W2033989457 cites W2100917733 @default.
• W2033989457 cites W2119446397 @default.
• W2033989457 cites W2139352977 @default.
• W2033989457 cites W2148765364 @default.
• W2033989457 cites W2148927531 @default.
• W2033989457 cites W2149759706 @default.
• W2033989457 cites W4230175933 @default.
• W2033989457 cites W4249438895 @default.
• W2033989457 cites W4253530158 @default.
• W2033989457 cites W4322388192 @default.
• W2033989457 doi "https://doi.org/10.1002/chem.200601624" @default.
• W2033989457 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17407110" @default.
• W2033989457 hasPublicationYear "2007" @default.
• W2033989457 type Work @default.
• W2033989457 sameAs 2033989457 @default.
• W2033989457 citedByCount "50" @default.
• W2033989457 countsByYear W20339894572012 @default.
• W2033989457 countsByYear W20339894572013 @default.
• W2033989457 countsByYear W20339894572014 @default.
• W2033989457 countsByYear W20339894572015 @default.
• W2033989457 countsByYear W20339894572016 @default.
• W2033989457 countsByYear W20339894572017 @default.
• W2033989457 countsByYear W20339894572018 @default.
• W2033989457 countsByYear W20339894572019 @default.
• W2033989457 countsByYear W20339894572020 @default.
• W2033989457 countsByYear W20339894572021 @default.
• W2033989457 countsByYear W20339894572022 @default.
• W2033989457 crossrefType "journal-article" @default.
• W2033989457 hasAuthorship W2033989457A5012535610 @default.
• W2033989457 hasAuthorship W2033989457A5051065845 @default.
• W2033989457 hasAuthorship W2033989457A5060002817 @default.
• W2033989457 hasAuthorship W2033989457A5081325802 @default.
• W2033989457 hasConcept C111368507 @default.
• W2033989457 hasConcept C113196181 @default.
• W2033989457 hasConcept C127313418 @default.
• W2033989457 hasConcept C127413603 @default.
• W2033989457 hasConcept C150394285 @default.
• W2033989457 hasConcept C171250308 @default.
• W2033989457 hasConcept C176406525 @default.
• W2033989457 hasConcept C178790620 @default.
• W2033989457 hasConcept C185592680 @default.
• W2033989457 hasConcept C192562407 @default.
• W2033989457 hasConcept C204628709 @default.
• W2033989457 hasConcept C2776190903 @default.
• W2033989457 hasConcept C2777289219 @default.
• W2033989457 hasConcept C2778204606 @default.
• W2033989457 hasConcept C42360764 @default.
• W2033989457 hasConcept C43617362 @default.
• W2033989457 hasConcept C55493867 @default.
• W2033989457 hasConcept C7070889 @default.
• W2033989457 hasConcept C73826308 @default.
• W2033989457 hasConcept C8673954 @default.
• W2033989457 hasConcept C88001094 @default.
• W2033989457 hasConceptScore W2033989457C111368507 @default.
• W2033989457 hasConceptScore W2033989457C113196181 @default.
• W2033989457 hasConceptScore W2033989457C127313418 @default.
• W2033989457 hasConceptScore W2033989457C127413603 @default.
• W2033989457 hasConceptScore W2033989457C150394285 @default.
• W2033989457 hasConceptScore W2033989457C171250308 @default.

• next