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• W2033994422 abstract "Polymerase chain reaction (PCR) amplification of full-length envelope genes from the human immunodeficiency virus type 1 (HIV-1) directly from uncultured clinical samples is difficult. This paper describes a comparative assessment of the performance of three thermostable polymerases in an HIV-1 full-length envelope gene PCR. The PCR method utilising Expand HiFi polymerase was successful when using DNA samples extracted from a variety of sources including blood, semen and various tissues. This method generated high and specific yields of product from samples containing as little as one copy of HIV-1 proviral DNA. The resulting PCR products were suitable for a variety of downstream analytical methods including DNA sequence analysis." @default.
• W2033994422 created "2016-06-24" @default.
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• W2033994422 date "2000-07-01" @default.
• W2033994422 modified "2023-09-26" @default.
• W2033994422 title "A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus" @default.
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• W2033994422 doi "https://doi.org/10.1016/s0166-0934(00)00179-8" @default.
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