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• W2033996401 abstract "A high-sensitivity assay has been developed for the detection of human topoisomerase I with single molecule resolution. The method uses magnetic sepharose beads to concentrate rolling circle products, produced by the amplification of DNA molecules circularized by topoisomerase I and detectable with a confocal microscope as single and discrete dots, once reacted with fluorescent probes. Each dot, corresponding to a single cleavage–religation event mediated by the enzyme, can be counted due to its high signal/noise ratio, allowing detection of 0.3 pM enzyme and representing a valid method to detect the enzyme activity in highly diluted samples." @default.
• W2033996401 created "2016-06-24" @default.
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• W2033996401 date "2014-04-01" @default.
• W2033996401 modified "2023-09-27" @default.
• W2033996401 title "Rolling circle amplification-based detection of human topoisomerase I activity on magnetic beads" @default.
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• W2033996401 doi "https://doi.org/10.1016/j.ab.2014.02.003" @default.
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