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- W2034002664 abstract "Procedures for the isolation of vacuoles from the first leaf of oats at different stages of development were compared as to yield, size and purity of the vacuoles isolated. Mechanical disruption or polybase-induced lysis of isolated protoplasts did not lead to clean vacuole preparations. In contrast, relatively pure vacuoles were obtained by phosphate-dependent osmotic lysis. Liberation of intact vacuoles required a minimum concentration of K2HPO4 in the lysis medium, lower concentrations leading to vacuole fragmentation. The maximum concentration by which complete lysis occurred decreased progressively when leaves were fully grown and started to senesce. Only vacuoplasts (vacuoles with adhering cytoplasm) could be obtained from leaves older than 17 days. The implications for the control of senescence in oat leaves are discussed." @default.
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- W2034002664 title "Isolation of vacuoles from developing oat leaves" @default.
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- W2034002664 doi "https://doi.org/10.1016/0168-9452(87)90049-5" @default.
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