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• W2034004577 abstract "Many papers in the literature have described complex effects of flavonoids and other polyphenols on cells in culture. In this paper we show that hydroxytyrosol, delphinidin chloride and rosmarinic acid are unstable in three commonly-used cell culture media (Dulbecco’s modified Eagle’s medium (DMEM), RPMI 1640 (RPMI) and Minimal Essential Medium Eagle (MEM)) and undergo rapid oxidation to generate H2O2. This may have confounded some previous studies on the cellular effects of these compounds. By contrast, apigenin, curcumin, hesperetin, naringenin, resveratrol and tyrosol did not generate significant H2O2 levels in these media. Nevertheless, curcumin and, to a lesser extent, resveratrol (but not tyrosol) were also unstable in DMEM, so the absence of detectable H2O2 production by a compound in cell culture media should not be equated to stability of that compound. Compound instability and generation of H2O2 must be taken into account in interpreting effects of phenolic compounds on cells in culture." @default.
• W2034004577 created "2016-06-24" @default.
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• W2034004577 date "2010-09-01" @default.
• W2034004577 modified "2023-09-29" @default.
• W2034004577 title "Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media" @default.
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• W2034004577 doi "https://doi.org/10.1016/j.abb.2010.06.012" @default.
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