FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034005389> ?p ?o ?g. }

• W2034005389 endingPage "328" @default.
• W2034005389 startingPage "324" @default.
• W2034005389 abstract "The adsorption of the protein avidin from hen egg white on patterns of silicon dioxide and platinum surfaces on a microchip and the use of fluorescent microscopy to detect binding of biotin are described. A silicon dioxide microchip was formed using plasma-enhanced chemical vapor deposition while platinum was deposited using radiofrequency sputtering. After cleaning using a plasma arc, the chips were placed into solutions containing avidin or bovine serum albumin. The avidin was adsorbed onto the microchips from phosphate-buffered saline (PBS) or from PBS to which ammonium sulfate had been added. Avidin was also adsorbed onto bovine serum albumin (BSA)-coated surfaces of oxide and platinum. Fluorescence microscopy was used to confirm adsorption of labeled protein, or the binding of fluorescently labeled biotin onto previously adsorbed, unlabeled avidin. When labeled biotin in PBS was presented to avidin adsorbed onto a BSA-coated microchip, the fluorescence signal was significantly higher than for avidin adsorbed onto the biochip alone. The results show that a simple, low-cost adsorption process can deposit active protein onto a chip in an approach that has potential application in the development of protein biochips for the detection of biological species. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 324–328, 2001." @default.
• W2034005389 created "2016-06-24" @default.
• W2034005389 creator A5018368779 @default.
• W2034005389 creator A5026552079 @default.
• W2034005389 creator A5050715203 @default.
• W2034005389 creator A5053912700 @default.
• W2034005389 creator A5066888603 @default.
• W2034005389 creator A5068782564 @default.
• W2034005389 date "2001-01-01" @default.
• W2034005389 modified "2023-10-17" @default.
• W2034005389 title "Adsorption of avidin on microfabricated surfaces for protein biochip applications" @default.
• W2034005389 cites W1586085231 @default.
• W2034005389 cites W1990246037 @default.
• W2034005389 cites W2008101397 @default.
• W2034005389 cites W2009421388 @default.
• W2034005389 cites W2023217821 @default.
• W2034005389 cites W2029320367 @default.
• W2034005389 cites W2029456980 @default.
• W2034005389 cites W2069829737 @default.
• W2034005389 cites W2081710345 @default.
• W2034005389 cites W2081765895 @default.
• W2034005389 cites W2113492577 @default.
• W2034005389 cites W2159511216 @default.
• W2034005389 doi "https://doi.org/10.1002/bit.1065" @default.
• W2034005389 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11283915" @default.
• W2034005389 hasPublicationYear "2001" @default.
• W2034005389 type Work @default.
• W2034005389 sameAs 2034005389 @default.
• W2034005389 citedByCount "48" @default.
• W2034005389 countsByYear W20340053892012 @default.
• W2034005389 countsByYear W20340053892013 @default.
• W2034005389 countsByYear W20340053892014 @default.
• W2034005389 countsByYear W20340053892015 @default.
• W2034005389 countsByYear W20340053892016 @default.
• W2034005389 countsByYear W20340053892018 @default.
• W2034005389 countsByYear W20340053892019 @default.
• W2034005389 countsByYear W20340053892023 @default.
• W2034005389 crossrefType "journal-article" @default.
• W2034005389 hasAuthorship W2034005389A5018368779 @default.
• W2034005389 hasAuthorship W2034005389A5026552079 @default.
• W2034005389 hasAuthorship W2034005389A5050715203 @default.
• W2034005389 hasAuthorship W2034005389A5053912700 @default.
• W2034005389 hasAuthorship W2034005389A5066888603 @default.
• W2034005389 hasAuthorship W2034005389A5068782564 @default.
• W2034005389 hasConcept C121332964 @default.
• W2034005389 hasConcept C150394285 @default.
• W2034005389 hasConcept C160756335 @default.
• W2034005389 hasConcept C161790260 @default.
• W2034005389 hasConcept C169274487 @default.
• W2034005389 hasConcept C171250308 @default.
• W2034005389 hasConcept C176406525 @default.
• W2034005389 hasConcept C178790620 @default.
• W2034005389 hasConcept C185592680 @default.
• W2034005389 hasConcept C191897082 @default.
• W2034005389 hasConcept C192562407 @default.
• W2034005389 hasConcept C2778204606 @default.
• W2034005389 hasConcept C2779089622 @default.
• W2034005389 hasConcept C43617362 @default.
• W2034005389 hasConcept C518104683 @default.
• W2034005389 hasConcept C55493867 @default.
• W2034005389 hasConcept C62520636 @default.
• W2034005389 hasConcept C86807702 @default.
• W2034005389 hasConcept C87892846 @default.
• W2034005389 hasConcept C88001094 @default.
• W2034005389 hasConcept C91881484 @default.
• W2034005389 hasConceptScore W2034005389C121332964 @default.
• W2034005389 hasConceptScore W2034005389C150394285 @default.
• W2034005389 hasConceptScore W2034005389C160756335 @default.
• W2034005389 hasConceptScore W2034005389C161790260 @default.
• W2034005389 hasConceptScore W2034005389C169274487 @default.
• W2034005389 hasConceptScore W2034005389C171250308 @default.
• W2034005389 hasConceptScore W2034005389C176406525 @default.
• W2034005389 hasConceptScore W2034005389C178790620 @default.
• W2034005389 hasConceptScore W2034005389C185592680 @default.
• W2034005389 hasConceptScore W2034005389C191897082 @default.
• W2034005389 hasConceptScore W2034005389C192562407 @default.
• W2034005389 hasConceptScore W2034005389C2778204606 @default.
• W2034005389 hasConceptScore W2034005389C2779089622 @default.
• W2034005389 hasConceptScore W2034005389C43617362 @default.
• W2034005389 hasConceptScore W2034005389C518104683 @default.
• W2034005389 hasConceptScore W2034005389C55493867 @default.
• W2034005389 hasConceptScore W2034005389C62520636 @default.
• W2034005389 hasConceptScore W2034005389C86807702 @default.
• W2034005389 hasConceptScore W2034005389C87892846 @default.
• W2034005389 hasConceptScore W2034005389C88001094 @default.
• W2034005389 hasConceptScore W2034005389C91881484 @default.
• W2034005389 hasIssue "4" @default.
• W2034005389 hasLocation W20340053891 @default.
• W2034005389 hasLocation W20340053892 @default.
• W2034005389 hasOpenAccess W2034005389 @default.
• W2034005389 hasPrimaryLocation W20340053891 @default.
• W2034005389 hasRelatedWork W1491713528 @default.
• W2034005389 hasRelatedWork W1794438978 @default.
• W2034005389 hasRelatedWork W2000274299 @default.
• W2034005389 hasRelatedWork W2009843049 @default.
• W2034005389 hasRelatedWork W2018430038 @default.
• W2034005389 hasRelatedWork W2052203724 @default.
• W2034005389 hasRelatedWork W2075319583 @default.

• next