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- W2034007926 abstract "To investigate the strand preference of the initial cleavage of human pre-miRNAs by human Dicer in vitro. We used a series of in vitro transcribed pre-miRNAs that were radioactively labeled at their 5′ or 3′ ends in cleavage reactions with recombinant human Dicer or HeLa cytoplasmic S100 extracts. Pre-miRNAs samples were purified by denaturing and native PAGE and only the stem-loop structures were used in the experiments. Products of cleavage reactions were resolved by denaturing PAGE, and scanned by phosphor-imaging. Recombinant hDicer performs a biased first-cleavage in the pre-let-7b and hsa-pre-miR-17 3′ strand. This result is recapitulated in HeLa S100 cytoplasmic extracts. The differential first-nick is observed in cleavage reactions only when stem-loops are substrates for hDicer." @default.
- W2034007926 created "2016-06-24" @default.
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- W2034007926 date "2009-08-01" @default.
- W2034007926 modified "2023-10-04" @default.
- W2034007926 title "First step in pre-miRNAs processing by human Dicer" @default.
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- W2034007926 doi "https://doi.org/10.1038/aps.2009.108" @default.
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