FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034016620> ?p ?o ?g. }

• W2034016620 endingPage "198" @default.
• W2034016620 startingPage "187" @default.
• W2034016620 abstract "Serum-free culture conditions that permit the continuous growth of A-431 human epidermoid carcinoma cells were developed. In Dulbecco's modified Eagle's synthetic nutritional medium (DME) supplemented with fetuin, insulin, transferrin, biotin, and oleic acid-fatty acid-free bovine serum albumin complex A-431 cells grew at a rate comparable to that observed in the presence of calf or fetal calf serum. Of the factors tested, oleic acid had the most pronounced stimulatory effect on the growth and [3H]-thymidine incorporation of A-431 cells in serum-free medium. A-431 cells have a high number of receptors for epidermal growth factor (EGF); they bind and rapidly internalize EGF. Nevertheless, EGF did not stimulate either the growth or the [3H]-thymidine incorporation of these cells. Analyses of [125I]-EGF binding data indicated that A-431 cells grown in the presence of calf serum had about 3.2-3.9 X 10(6) specific, saturable EGF receptor sites on their surface. Linear Scatchard plots indicated a single class of noninteracting receptors with an apparent equilibrium dissociation constant of about 2.8 X 10(-9) M. The average number of receptors of A-431 cells maintained in DME supplemented with only fetuin, insulin, and transferrin for several months was significantly less, 1.54 X 10(6), than that of A-431 stock cells cultured in the same medium for 2 days only (2.68 X 10(6)). The apparent dissociation constants for the same cell populations were, however, similar, 4.5 X 10(-9) M and 4.1 X 10(-9) M, respectively. Stimulation of growth by oleic acid resulted in about 20% decrease in the average number of receptor sites, with an increase in the apparent equilibrium dissociation constant." @default.
• W2034016620 created "2016-06-24" @default.
• W2034016620 creator A5020185466 @default.
• W2034016620 creator A5085745814 @default.
• W2034016620 date "1982-10-01" @default.
• W2034016620 modified "2023-09-24" @default.
• W2034016620 title "Culture of A-431 human epidermoid carcinoma cells in serum-free medium: Effect of culture conditions on the binding of [125I]-epidermal growth factor" @default.
• W2034016620 cites W1196484601 @default.
• W2034016620 cites W1262734359 @default.
• W2034016620 cites W1496974366 @default.
• W2034016620 cites W1498071142 @default.
• W2034016620 cites W1501383613 @default.
• W2034016620 cites W1522098040 @default.
• W2034016620 cites W1524333424 @default.
• W2034016620 cites W1548966243 @default.
• W2034016620 cites W1600133329 @default.
• W2034016620 cites W1606919023 @default.
• W2034016620 cites W1894641937 @default.
• W2034016620 cites W1970862733 @default.
• W2034016620 cites W1978868657 @default.
• W2034016620 cites W1979224484 @default.
• W2034016620 cites W1983649348 @default.
• W2034016620 cites W1985749328 @default.
• W2034016620 cites W1987190223 @default.
• W2034016620 cites W1991274673 @default.
• W2034016620 cites W1994341785 @default.
• W2034016620 cites W1998599114 @default.
• W2034016620 cites W2005668137 @default.
• W2034016620 cites W2006323812 @default.
• W2034016620 cites W2010394496 @default.
• W2034016620 cites W2015080723 @default.
• W2034016620 cites W2032714210 @default.
• W2034016620 cites W2033280477 @default.
• W2034016620 cites W2035604873 @default.
• W2034016620 cites W2035783270 @default.
• W2034016620 cites W2039450453 @default.
• W2034016620 cites W2040469480 @default.
• W2034016620 cites W2045372146 @default.
• W2034016620 cites W2048004078 @default.
• W2034016620 cites W2063876611 @default.
• W2034016620 cites W2064331161 @default.
• W2034016620 cites W2064762154 @default.
• W2034016620 cites W2074511156 @default.
• W2034016620 cites W2089506208 @default.
• W2034016620 cites W2099335992 @default.
• W2034016620 cites W2102126511 @default.
• W2034016620 cites W2109790916 @default.
• W2034016620 cites W2114627940 @default.
• W2034016620 cites W2149108074 @default.
• W2034016620 cites W2169991926 @default.
• W2034016620 cites W2323246601 @default.
• W2034016620 cites W33564209 @default.
• W2034016620 cites W4241786181 @default.
• W2034016620 doi "https://doi.org/10.1002/aja.1001650207" @default.
• W2034016620 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6983291" @default.
• W2034016620 hasPublicationYear "1982" @default.
• W2034016620 type Work @default.
• W2034016620 sameAs 2034016620 @default.
• W2034016620 citedByCount "7" @default.
• W2034016620 countsByYear W20340166202012 @default.
• W2034016620 crossrefType "journal-article" @default.
• W2034016620 hasAuthorship W2034016620A5020185466 @default.
• W2034016620 hasAuthorship W2034016620A5085745814 @default.
• W2034016620 hasConcept C108625454 @default.
• W2034016620 hasConcept C126322002 @default.
• W2034016620 hasConcept C134018914 @default.
• W2034016620 hasConcept C1491633281 @default.
• W2034016620 hasConcept C153911025 @default.
• W2034016620 hasConcept C165616093 @default.
• W2034016620 hasConcept C170493617 @default.
• W2034016620 hasConcept C22814914 @default.
• W2034016620 hasConcept C2775960820 @default.
• W2034016620 hasConcept C2776362946 @default.
• W2034016620 hasConcept C2777546739 @default.
• W2034016620 hasConcept C2778541144 @default.
• W2034016620 hasConcept C2779404806 @default.
• W2034016620 hasConcept C2994460389 @default.
• W2034016620 hasConcept C54355233 @default.
• W2034016620 hasConcept C55493867 @default.
• W2034016620 hasConcept C71924100 @default.
• W2034016620 hasConcept C74998103 @default.
• W2034016620 hasConcept C81885089 @default.
• W2034016620 hasConcept C86803240 @default.
• W2034016620 hasConcept C86807702 @default.
• W2034016620 hasConceptScore W2034016620C108625454 @default.
• W2034016620 hasConceptScore W2034016620C126322002 @default.
• W2034016620 hasConceptScore W2034016620C134018914 @default.
• W2034016620 hasConceptScore W2034016620C1491633281 @default.
• W2034016620 hasConceptScore W2034016620C153911025 @default.
• W2034016620 hasConceptScore W2034016620C165616093 @default.
• W2034016620 hasConceptScore W2034016620C170493617 @default.
• W2034016620 hasConceptScore W2034016620C22814914 @default.
• W2034016620 hasConceptScore W2034016620C2775960820 @default.
• W2034016620 hasConceptScore W2034016620C2776362946 @default.
• W2034016620 hasConceptScore W2034016620C2777546739 @default.
• W2034016620 hasConceptScore W2034016620C2778541144 @default.
• W2034016620 hasConceptScore W2034016620C2779404806 @default.
• W2034016620 hasConceptScore W2034016620C2994460389 @default.

• next