FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034018495> ?p ?o ?g. }

• W2034018495 endingPage "4908" @default.
• W2034018495 startingPage "4898" @default.
• W2034018495 abstract "This study shows that MP-1, a peptide from the venom of the Polybia paulista wasp, is more toxic to human leukemic T-lymphocytes than to human primary lymphocytes. By using model membranes and electrophysiology measurements to investigate the molecular mechanisms underlying this selective action, the porelike activity of MP-1 was identified with several bilayer compositions. The highest average conductance was found in bilayers formed by phosphatidylcholine or a mixture of phosphatidylcholine and phosphatidylserine (70:30). The presence of cholesterol or cardiolipin substantially decreases the MP-1 pore activity, suggesting that the membrane fluidity influences the mechanism of selective toxicity. The determination of partition coefficients from the anisotropy of Trp indicated higher coefficients for the anionic bilayers. The partition coefficients were found to be 1 order of magnitude smaller when the bilayers contain cholesterol or a mixture of cholesterol and sphingomyelin. The blue shift fluorescence, anisotropy values, and Stern-Volmer constants are indications of a deeper penetration of MP-1 into anionic bilayers than into zwitterionic bilayers. Our results indicate that MP-1 prefers to target leukemic cell membranes, and its toxicity is probably related to the induction of necrosis and not to DNA fragmentation. This mode of action can be interpreted considering a number of bilayer properties like fluidity, lipid charge, and domain formation. Cholesterol-containing bilayers are less fluid and less charged and have a tendency to form domains. In comparison to healthy cells, leukemic T-lymphocyte membranes are deprived of this lipid, resulting in decreased peptide binding and lower conductance. We showed that the higher content of anionic lipids increases the level of binding of the peptide to bilayers. Additionally, the absence of cholesterol resulted in enhanced pore activity. These findings may drive the selective toxicity of MP-1 to Jurkat cells." @default.
• W2034018495 created "2016-06-24" @default.
• W2034018495 creator A5004080311 @default.
• W2034018495 creator A5004856738 @default.
• W2034018495 creator A5016616324 @default.
• W2034018495 creator A5019832494 @default.
• W2034018495 creator A5042602516 @default.
• W2034018495 creator A5051137933 @default.
• W2034018495 creator A5053295842 @default.
• W2034018495 creator A5060386615 @default.
• W2034018495 creator A5082057360 @default.
• W2034018495 date "2012-06-06" @default.
• W2034018495 modified "2023-10-03" @default.
• W2034018495 title "Influence of the Bilayer Composition on the Binding and Membrane Disrupting Effect of Polybia-MP1, an Antimicrobial Mastoparan Peptide with Leukemic T-Lymphocyte Cell Selectivity" @default.
• W2034018495 cites W149428333 @default.
• W2034018495 cites W1513290101 @default.
• W2034018495 cites W1965400499 @default.
• W2034018495 cites W1967817108 @default.
• W2034018495 cites W1978730586 @default.
• W2034018495 cites W1978747518 @default.
• W2034018495 cites W1979129905 @default.
• W2034018495 cites W1979212779 @default.
• W2034018495 cites W1980343860 @default.
• W2034018495 cites W1982834306 @default.
• W2034018495 cites W1989451255 @default.
• W2034018495 cites W1994446262 @default.
• W2034018495 cites W1997822065 @default.
• W2034018495 cites W1997993218 @default.
• W2034018495 cites W2003538316 @default.
• W2034018495 cites W2009300453 @default.
• W2034018495 cites W2012983247 @default.
• W2034018495 cites W2013927144 @default.
• W2034018495 cites W2019518993 @default.
• W2034018495 cites W2023703024 @default.
• W2034018495 cites W2026821359 @default.
• W2034018495 cites W2031227547 @default.
• W2034018495 cites W2038276423 @default.
• W2034018495 cites W2050428252 @default.
• W2034018495 cites W2064451603 @default.
• W2034018495 cites W2065196912 @default.
• W2034018495 cites W2073097129 @default.
• W2034018495 cites W2074085543 @default.
• W2034018495 cites W2075518645 @default.
• W2034018495 cites W2089593580 @default.
• W2034018495 cites W2110052595 @default.
• W2034018495 cites W2110178738 @default.
• W2034018495 cites W2120569780 @default.
• W2034018495 cites W2129995310 @default.
• W2034018495 cites W2152717577 @default.
• W2034018495 cites W2158438453 @default.
• W2034018495 cites W2171527056 @default.
• W2034018495 cites W2316680848 @default.
• W2034018495 doi "https://doi.org/10.1021/bi201608d" @default.
• W2034018495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22630563" @default.
• W2034018495 hasPublicationYear "2012" @default.
• W2034018495 type Work @default.
• W2034018495 sameAs 2034018495 @default.
• W2034018495 citedByCount "36" @default.
• W2034018495 countsByYear W20340184952013 @default.
• W2034018495 countsByYear W20340184952014 @default.
• W2034018495 countsByYear W20340184952015 @default.
• W2034018495 countsByYear W20340184952016 @default.
• W2034018495 countsByYear W20340184952017 @default.
• W2034018495 countsByYear W20340184952018 @default.
• W2034018495 countsByYear W20340184952019 @default.
• W2034018495 countsByYear W20340184952020 @default.
• W2034018495 countsByYear W20340184952021 @default.
• W2034018495 countsByYear W20340184952022 @default.
• W2034018495 countsByYear W20340184952023 @default.
• W2034018495 crossrefType "journal-article" @default.
• W2034018495 hasAuthorship W2034018495A5004080311 @default.
• W2034018495 hasAuthorship W2034018495A5004856738 @default.
• W2034018495 hasAuthorship W2034018495A5016616324 @default.
• W2034018495 hasAuthorship W2034018495A5019832494 @default.
• W2034018495 hasAuthorship W2034018495A5042602516 @default.
• W2034018495 hasAuthorship W2034018495A5051137933 @default.
• W2034018495 hasAuthorship W2034018495A5053295842 @default.
• W2034018495 hasAuthorship W2034018495A5060386615 @default.
• W2034018495 hasAuthorship W2034018495A5082057360 @default.
• W2034018495 hasConcept C111357860 @default.
• W2034018495 hasConcept C122398194 @default.
• W2034018495 hasConcept C12554922 @default.
• W2034018495 hasConcept C185592680 @default.
• W2034018495 hasConcept C192157962 @default.
• W2034018495 hasConcept C2776330855 @default.
• W2034018495 hasConcept C2778918659 @default.
• W2034018495 hasConcept C2779281246 @default.
• W2034018495 hasConcept C39944091 @default.
• W2034018495 hasConcept C41625074 @default.
• W2034018495 hasConcept C55493867 @default.
• W2034018495 hasConcept C61322309 @default.
• W2034018495 hasConcept C86803240 @default.
• W2034018495 hasConceptScore W2034018495C111357860 @default.
• W2034018495 hasConceptScore W2034018495C122398194 @default.
• W2034018495 hasConceptScore W2034018495C12554922 @default.
• W2034018495 hasConceptScore W2034018495C185592680 @default.
• W2034018495 hasConceptScore W2034018495C192157962 @default.
• W2034018495 hasConceptScore W2034018495C2776330855 @default.

• next