Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034018713> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2034018713 endingPage "111" @default.
- W2034018713 startingPage "105" @default.
- W2034018713 abstract "In previous studies, we have cloned and sequenced a 5'-end region of the multidrug resistance-associated protein (MRP) gene that contains promoter activity as assessed through transient transfections of constructs contained in a pCAT basic reporter plasmid. In the present study, using a series of deletion mutants, evidence was obtained that the SP1 binding sites contained in the promoter are essential for optimal MRP transcriptional activity. These results were supported by the finding that introduction of site-specific mutations into the wild-type SP1 sequence produced a major reduction in CAT activity. DNase I protection assays also demonstrated that SP1 sites are protected from hydrolysis with proteins from nuclei of a variety of cell lines. Gel mobility-shift assays with proteins extracted from CHO, HeLa, HL60, or HL60/ADR demonstrated the presence of a protein that bound to the wild-type SP1 sequence but not to an SP1 sequence containing site-specific mutations. The mobility shift with nuclear extracts was closely similar to that occurring after incubating purified SP1 protein with wild-type SP1 sequence. DNA supershift experiments with antibody to SP1 strongly suggest that the complexes formed with nuclear extracts contain the SP1 protein." @default.
- W2034018713 created "2016-06-24" @default.
- W2034018713 creator A5083956730 @default.
- W2034018713 date "1996-02-01" @default.
- W2034018713 modified "2023-10-12" @default.
- W2034018713 title "Evidence that SP1 Modulates Transcriptional Activity of the Multidrug Resistance-Associated Protein Gene" @default.
- W2034018713 cites W1497498736 @default.
- W2034018713 cites W1502426706 @default.
- W2034018713 cites W1539776437 @default.
- W2034018713 cites W1547452419 @default.
- W2034018713 cites W1993243592 @default.
- W2034018713 cites W2000662019 @default.
- W2034018713 cites W2039560640 @default.
- W2034018713 cites W2079882118 @default.
- W2034018713 cites W2080736726 @default.
- W2034018713 cites W2097878215 @default.
- W2034018713 cites W2118234018 @default.
- W2034018713 cites W2138270253 @default.
- W2034018713 cites W2154846669 @default.
- W2034018713 cites W2155342110 @default.
- W2034018713 cites W2168070336 @default.
- W2034018713 cites W2168095256 @default.
- W2034018713 cites W2466100037 @default.
- W2034018713 doi "https://doi.org/10.1089/dna.1996.15.105" @default.
- W2034018713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8634138" @default.
- W2034018713 hasPublicationYear "1996" @default.
- W2034018713 type Work @default.
- W2034018713 sameAs 2034018713 @default.
- W2034018713 citedByCount "43" @default.
- W2034018713 countsByYear W20340187132012 @default.
- W2034018713 countsByYear W20340187132014 @default.
- W2034018713 countsByYear W20340187132018 @default.
- W2034018713 countsByYear W20340187132022 @default.
- W2034018713 crossrefType "journal-article" @default.
- W2034018713 hasAuthorship W2034018713A5083956730 @default.
- W2034018713 hasConcept C101762097 @default.
- W2034018713 hasConcept C104317684 @default.
- W2034018713 hasConcept C143065580 @default.
- W2034018713 hasConcept C150194340 @default.
- W2034018713 hasConcept C153911025 @default.
- W2034018713 hasConcept C161733203 @default.
- W2034018713 hasConcept C22744801 @default.
- W2034018713 hasConcept C55493867 @default.
- W2034018713 hasConcept C57467964 @default.
- W2034018713 hasConcept C86803240 @default.
- W2034018713 hasConceptScore W2034018713C101762097 @default.
- W2034018713 hasConceptScore W2034018713C104317684 @default.
- W2034018713 hasConceptScore W2034018713C143065580 @default.
- W2034018713 hasConceptScore W2034018713C150194340 @default.
- W2034018713 hasConceptScore W2034018713C153911025 @default.
- W2034018713 hasConceptScore W2034018713C161733203 @default.
- W2034018713 hasConceptScore W2034018713C22744801 @default.
- W2034018713 hasConceptScore W2034018713C55493867 @default.
- W2034018713 hasConceptScore W2034018713C57467964 @default.
- W2034018713 hasConceptScore W2034018713C86803240 @default.
- W2034018713 hasIssue "2" @default.
- W2034018713 hasLocation W20340187131 @default.
- W2034018713 hasLocation W20340187132 @default.
- W2034018713 hasOpenAccess W2034018713 @default.
- W2034018713 hasPrimaryLocation W20340187131 @default.
- W2034018713 hasRelatedWork W1984664666 @default.
- W2034018713 hasRelatedWork W2114174502 @default.
- W2034018713 hasRelatedWork W2114870167 @default.
- W2034018713 hasRelatedWork W2155835698 @default.
- W2034018713 hasRelatedWork W2403366211 @default.
- W2034018713 hasRelatedWork W2429129060 @default.
- W2034018713 hasRelatedWork W2750385014 @default.
- W2034018713 hasRelatedWork W3123442693 @default.
- W2034018713 hasRelatedWork W2409844362 @default.
- W2034018713 hasRelatedWork W2908588478 @default.
- W2034018713 hasVolume "15" @default.
- W2034018713 isParatext "false" @default.
- W2034018713 isRetracted "false" @default.
- W2034018713 magId "2034018713" @default.
- W2034018713 workType "article" @default.