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• W2034039741 abstract "A collagen-glycosaminoglycan sponge composed of collagen (80%), chondroitin-4-sulfate (13.3%) and heparan sulfate (6.6%) was cross-linked using the acyl azide methode or glutaraldehyde (0.0075%). Under optimal conditions, the denaturation temperature (Td) was raised to 69 °C (+ 23 °C) for the sponge treated by the acyl azide method and to 68 °C (+ 22 °C) for that treated with glutaraldehyde. The biocompatibility of the treated and control sponges was studied up to 3 months after subcutaneous implantation in rats by analysing cellular responses and calcification by histological and ultrastructural methods. A control collagen-glycosaminoglycan sponge was rapidly invaded by mononuclear cells (8 days), with the formation of granulation tissue. Calcification was observed at the periphery of the implant after 8 days, and the implant was entirely calcified after 15 days; it was degraded progressively after 30 days. Acyl azide treatment increased the persistence of the sponge in vivo up to 90 days and inhibited its calcification. A glutaraldehyde-treated sponge was completely calcified after 15 days, and calcified nodules persisted after 90 days. Thus, acyl azide method efficiently cross-linked a collagenglycosaminoglycan sponge and inhibited calcification after subcutaneous implantation in rats (at least up to 90 days after implantation)." @default.
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• W2034039741 date "1992-08-01" @default.
• W2034039741 modified "2023-10-11" @default.
• W2034039741 title "Inhibition of Calcification In Vivo by Acyl Azide Cross-Linking of a Collagen-Glycosarninoglycan Sponge" @default.
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• W2034039741 doi "https://doi.org/10.1016/s0934-8832(11)80078-8" @default.
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