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• W2034100663 abstract "The growth characteristics of the BM-5 insect cell line of Bombyx mori (silkworm) have been experimentally investigated in order to develop optimal growth protocols when these cells are used to produce large quantities of biopesticides or human proteins by recombinant baculoviruses. Experiments were performed in 2 mL wells and 200 mL spinner flasks. Spinner flasks were operated at 80 rpm with 0.3% methyl cellulose (MCL) added to the medium in order to protect the cells from liquid shear stress. In addition to the effect of agitation rate and amount of MCL added to the medium, the cell response during the adaptation to growth in suspension from stationary cultures is reported. Exposure of the cells to varying nutrient and metabolite concentrations is accomplished through batch and repeated-batch modes in 2 mL wells. The results imply that glutamine is a limiting nutrient and lactate has an inhibitory effect on cell growth. Ammonia depletion from the medium was accompanied by uric acid accumulation, suggesting that ammonia is converted to this metabolic product by the “uricotelic” and “nucleicolytic” metabolic pathways. On a étudié expérimentalement les caractéiistiques de croissance d'une lignée de cellules d'insectes BM-5 de Bombyx mori (ver aG soie) dans le but d'élaborer des protocoles de croissance optimale lorsque ces cellules sont utilisées pour produire d'importantes quantités de pesticides biologiques ou de protéines humaines par des baculovirus recombinants. Les expériences ont été réalisées dans des tubes de 2 ml et des flacons rotatifs de 200 ml. On a agité les flacons à 80 tours par minute avec 0,3 % de méthyl cellulose (MCL) pour protéger les cellules des contraintes de cisaillement liquides. En plus de l'effet de la vitesse d'agitation et de la quantité de MCL ajoutée au milieu, on indique la réponse des cellules durant l'adaptation à la croissance en suspension à partir de cultures stationnaires. L'exposition des cellules à des concentrations variables en éléments nutritifs et en métabolites est effectuée en mode discontinu ou en mode discontinu cyclique dans des tubes de 2 ml. Les résultats montrent que la glutamine est un élément nutritif limitant et que le lactate a un effet inhibiteur sur la croissance des cellules. L'élimination de l'ammoniaque du milieu s'accompagne d'une accumulation d'acide urique, ce qui suggère que l'ammoniaque est converti en produits métaboliques par des mécanismes métaboliques “uricotéliques” et “nucléicolytiques”." @default.
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• W2034100663 date "1991-04-01" @default.
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• W2034100663 title "Growth characteristics of a<i>Bombyx mori</i>insect cell line in stationary and suspension cultures" @default.
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• W2034100663 doi "https://doi.org/10.1002/cjce.5450690209" @default.
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