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- W2034105383 abstract "Early checking of the genetic stability of tissue culture–derived plants is necessary to obtain all the potential benefits of clonal forestry. Previous work in Quercus suber L. using random amplified polymorphic DNA (RAPD) markers showed no genetic variation among somatic embryos within embryogenic lines of zygotic origin. Genetic fingerprinting based on amplified fragment length polymorphisms (AFLP) allows the direct analysis of variation at the entire DNA level by generating more reproducible markers than RAPDs. To confirm the absence of genetic variation within six embryogenic lines of zygotic origin, six primer pairs were selected out of 48 combinations of primers for revealing up to 512 AFLP markers, 301 of them (58.8%) being polymorphic. The mean number of markers per genotype was 375. Again differences were recorded among embryogenic lines, even between those that arose from half‐sib zygotic embryos, but no variation was observed among somatic embryos within embryogenic lines. To check variation in..." @default.
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- W2034105383 date "2001-07-01" @default.
- W2034105383 modified "2023-09-26" @default.
- W2034105383 title "Early Checking of Genetic Stability of Cork Oak Somatic Embryos by AFLP Analysis" @default.
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- W2034105383 doi "https://doi.org/10.1086/320784" @default.
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