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• W2034114030 endingPage "5472" @default.
• W2034114030 startingPage "5464" @default.
• W2034114030 abstract "Detailed analysis of factors governing high affinity antibody−antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody D5 (which binds its target, “5-Helix”, with a reported KD of 50 pM). Combinatorial scanning mutagenesis libraries were prepared in which CDR residues on the D5 light chain were varied among WT side chain identity or alanine. Selection of these libraries against 5-Helix and then sequence analysis of the resulting population were used to quantify energetic consequences of mutation from wild-type to alanine (ΔΔGAla-WT) at each position. This analysis revealed several hotspot residues (ΔΔGAla-WT ≥ 1 kcal/mol) that formed combining site features critical to the affinity of the interaction. Tolerance of D5 light chain residues to alternative mutations was explored with a second library. We found that light chain residues located at the center and at the periphery of the D5 combining site contribute to shape complementarity and electrostatic characteristics. Thus, the affinity of D5 for 5-Helix arises from extended interactions involving both the heavy and light chains of D5. These results provide significant insight for future antibody engineering efforts." @default.
• W2034114030 created "2016-06-24" @default.
• W2034114030 creator A5030038763 @default.
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• W2034114030 date "2010-06-15" @default.
• W2034114030 modified "2023-09-28" @default.
• W2034114030 title "Contribution of Light Chain Residues to High Affinity Binding in an HIV-1 Antibody Explored by Combinatorial Scanning Mutagenesis" @default.
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• W2034114030 doi "https://doi.org/10.1021/bi100293q" @default.
• W2034114030 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2911358" @default.
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• W2034114030 hasPublicationYear "2010" @default.
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