Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034114423> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2034114423 endingPage "569" @default.
- W2034114423 startingPage "563" @default.
- W2034114423 abstract "Cell-free extracts with high 14 alpha-hydroxylase activity were prepared from induced vegetative cell cultures of Mucor piriformis by grinding in potassium phosphate buffer (0.05 M, pH 8.0) containing glucose (0.25 M), KCl (1 mM), glutathione (1.0 mM) and glycerol (10%). Although the ideal pH for preparing the cell-free extract from vegetative cells was 8.0, the pH optimum of the hydroxylase was found to be 7.6. Microsomes (2.0 mg) prepared from the crude cell-free extract hydroxylated progesterone to 14 alpha-hydroxyprogesterone in approximately 60% yields in 30 min in the presence of NADPH and O2. Microsomes prepared from the uninduced cells did not contain any 14 alpha-hydroxylase activity. The hydroxylase activity was inhibited to a significant extent by CO and p-chloromercuribenzoate whereas moderate inhibition was noticed in the presence of SKF-525A, metyrapone and N-methylmaleimide indicating the possible involvement of the cytochrome P-450 system in the reaction. The membrane bound hydroxylase was solubilized using Triton X-100 and the solubilized fraction contained nearly 35% of the original hydroxylase activity." @default.
- W2034114423 created "2016-06-24" @default.
- W2034114423 creator A5048683956 @default.
- W2034114423 creator A5089128720 @default.
- W2034114423 date "1993-06-01" @default.
- W2034114423 modified "2023-09-25" @default.
- W2034114423 title "Studies on the 14α-hydroxylation of progesterone in mucor piriformis" @default.
- W2034114423 cites W1775749144 @default.
- W2034114423 cites W1977461646 @default.
- W2034114423 cites W1997712374 @default.
- W2034114423 cites W1998783684 @default.
- W2034114423 cites W2031995413 @default.
- W2034114423 cites W2038068626 @default.
- W2034114423 cites W2045011898 @default.
- W2034114423 cites W2055117832 @default.
- W2034114423 cites W2062076128 @default.
- W2034114423 cites W2064345162 @default.
- W2034114423 cites W2083246216 @default.
- W2034114423 cites W2083783107 @default.
- W2034114423 cites W2091876394 @default.
- W2034114423 cites W2330215514 @default.
- W2034114423 cites W2433663193 @default.
- W2034114423 cites W3164072790 @default.
- W2034114423 cites W48185203 @default.
- W2034114423 doi "https://doi.org/10.1016/0960-0760(93)90173-t" @default.
- W2034114423 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8518211" @default.
- W2034114423 hasPublicationYear "1993" @default.
- W2034114423 type Work @default.
- W2034114423 sameAs 2034114423 @default.
- W2034114423 citedByCount "14" @default.
- W2034114423 countsByYear W20341144232012 @default.
- W2034114423 countsByYear W20341144232014 @default.
- W2034114423 crossrefType "journal-article" @default.
- W2034114423 hasAuthorship W2034114423A5048683956 @default.
- W2034114423 hasAuthorship W2034114423A5089128720 @default.
- W2034114423 hasConcept C134018914 @default.
- W2034114423 hasConcept C181199279 @default.
- W2034114423 hasConcept C185592680 @default.
- W2034114423 hasConcept C2776719974 @default.
- W2034114423 hasConcept C2780768313 @default.
- W2034114423 hasConcept C2781109383 @default.
- W2034114423 hasConcept C2781289165 @default.
- W2034114423 hasConcept C43617362 @default.
- W2034114423 hasConcept C55493867 @default.
- W2034114423 hasConcept C86803240 @default.
- W2034114423 hasConcept C87644729 @default.
- W2034114423 hasConceptScore W2034114423C134018914 @default.
- W2034114423 hasConceptScore W2034114423C181199279 @default.
- W2034114423 hasConceptScore W2034114423C185592680 @default.
- W2034114423 hasConceptScore W2034114423C2776719974 @default.
- W2034114423 hasConceptScore W2034114423C2780768313 @default.
- W2034114423 hasConceptScore W2034114423C2781109383 @default.
- W2034114423 hasConceptScore W2034114423C2781289165 @default.
- W2034114423 hasConceptScore W2034114423C43617362 @default.
- W2034114423 hasConceptScore W2034114423C55493867 @default.
- W2034114423 hasConceptScore W2034114423C86803240 @default.
- W2034114423 hasConceptScore W2034114423C87644729 @default.
- W2034114423 hasIssue "6" @default.
- W2034114423 hasLocation W20341144231 @default.
- W2034114423 hasLocation W20341144232 @default.
- W2034114423 hasOpenAccess W2034114423 @default.
- W2034114423 hasPrimaryLocation W20341144231 @default.
- W2034114423 hasRelatedWork W134341308 @default.
- W2034114423 hasRelatedWork W2008505849 @default.
- W2034114423 hasRelatedWork W2041294993 @default.
- W2034114423 hasRelatedWork W2046744055 @default.
- W2034114423 hasRelatedWork W2066470472 @default.
- W2034114423 hasRelatedWork W2067653855 @default.
- W2034114423 hasRelatedWork W2090723725 @default.
- W2034114423 hasRelatedWork W2399032410 @default.
- W2034114423 hasRelatedWork W2418057803 @default.
- W2034114423 hasRelatedWork W213664910 @default.
- W2034114423 hasVolume "45" @default.
- W2034114423 isParatext "false" @default.
- W2034114423 isRetracted "false" @default.
- W2034114423 magId "2034114423" @default.
- W2034114423 workType "article" @default.