Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034116338> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2034116338 endingPage "2461" @default.
- W2034116338 startingPage "2453" @default.
- W2034116338 abstract "Investigation of drug interactions between blood cells and endothelium is of high interest. The current study describes the development of a rapid fluorescence-based leukocyte transmigration system through endothelial cell monolayers for investigation of drug influences. To test the new assay, endothelial cells were cultured on microporous filters, pore size 3.0 microm, in 96-well-plates. Freshly isolated neutrophils were seeded on endothelial cell monolayers and transmigrated cells were measured after incubation for three hours. Migration of non-stimulated neutrophils through non-stimulated endothelial cell monolayer was used as control and set as 100%. The influence of the non-steroidal anti-inflammatory drug diclofenac was investigated. Assay precision tests were done using intraassay (within-day variability) and interassay (day-to-day variability) controls. Transmigration rate was decreased to 53 +/- 6.8% SD (diclofenac 0.7 microg/mL). Different concentrations showed a dose dependent effect (0.07 microg/mL: 97 +/- 9.5%, 7 microg/mL: 37 +/- 4.7%). Analysis of assay accuracy of the new 96-well-sized transmigration assay showed reliable results (coefficient of variation: intraassay 8.2 %; interassay 11.8%). In conclusion, this new, rapid, and sample saving 96-well-microtiter transmigration assay allows examination of drug influence on neutrophil migration through endothelial cell monolayers. Moreover, this assay can also be used for other cell-cell interactions." @default.
- W2034116338 created "2016-06-24" @default.
- W2034116338 creator A5004089125 @default.
- W2034116338 creator A5029196374 @default.
- W2034116338 creator A5029911477 @default.
- W2034116338 creator A5044747491 @default.
- W2034116338 creator A5066376515 @default.
- W2034116338 creator A5082163795 @default.
- W2034116338 date "1999-10-01" @default.
- W2034116338 modified "2023-09-23" @default.
- W2034116338 title "Rapid, fluorescence-based assay for microtiter plates to test drug influences on neutrophil transmigration through endothelial cell monolayers" @default.
- W2034116338 cites W1508597447 @default.
- W2034116338 cites W1587343486 @default.
- W2034116338 cites W1631426208 @default.
- W2034116338 cites W1778576079 @default.
- W2034116338 cites W1893947242 @default.
- W2034116338 cites W1970833034 @default.
- W2034116338 cites W1985413225 @default.
- W2034116338 cites W1985887823 @default.
- W2034116338 cites W1999876918 @default.
- W2034116338 cites W2003951221 @default.
- W2034116338 cites W2025826840 @default.
- W2034116338 cites W2032494769 @default.
- W2034116338 cites W2050904152 @default.
- W2034116338 cites W2051605568 @default.
- W2034116338 cites W2088073163 @default.
- W2034116338 cites W2092107787 @default.
- W2034116338 cites W2100404293 @default.
- W2034116338 cites W2140678674 @default.
- W2034116338 cites W2157603347 @default.
- W2034116338 cites W2312810899 @default.
- W2034116338 cites W2419211297 @default.
- W2034116338 cites W294674092 @default.
- W2034116338 doi "https://doi.org/10.1016/s0024-3205(99)00511-1" @default.
- W2034116338 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10622229" @default.
- W2034116338 hasPublicationYear "1999" @default.
- W2034116338 type Work @default.
- W2034116338 sameAs 2034116338 @default.
- W2034116338 citedByCount "2" @default.
- W2034116338 crossrefType "journal-article" @default.
- W2034116338 hasAuthorship W2034116338A5004089125 @default.
- W2034116338 hasAuthorship W2034116338A5029196374 @default.
- W2034116338 hasAuthorship W2034116338A5029911477 @default.
- W2034116338 hasAuthorship W2034116338A5044747491 @default.
- W2034116338 hasAuthorship W2034116338A5066376515 @default.
- W2034116338 hasAuthorship W2034116338A5082163795 @default.
- W2034116338 hasConcept C123012128 @default.
- W2034116338 hasConcept C134018914 @default.
- W2034116338 hasConcept C1491633281 @default.
- W2034116338 hasConcept C153911025 @default.
- W2034116338 hasConcept C185592680 @default.
- W2034116338 hasConcept C202751555 @default.
- W2034116338 hasConcept C2776992346 @default.
- W2034116338 hasConcept C2778810118 @default.
- W2034116338 hasConcept C43617362 @default.
- W2034116338 hasConcept C54355233 @default.
- W2034116338 hasConcept C55493867 @default.
- W2034116338 hasConcept C7070889 @default.
- W2034116338 hasConcept C81885089 @default.
- W2034116338 hasConcept C86803240 @default.
- W2034116338 hasConcept C98274493 @default.
- W2034116338 hasConceptScore W2034116338C123012128 @default.
- W2034116338 hasConceptScore W2034116338C134018914 @default.
- W2034116338 hasConceptScore W2034116338C1491633281 @default.
- W2034116338 hasConceptScore W2034116338C153911025 @default.
- W2034116338 hasConceptScore W2034116338C185592680 @default.
- W2034116338 hasConceptScore W2034116338C202751555 @default.
- W2034116338 hasConceptScore W2034116338C2776992346 @default.
- W2034116338 hasConceptScore W2034116338C2778810118 @default.
- W2034116338 hasConceptScore W2034116338C43617362 @default.
- W2034116338 hasConceptScore W2034116338C54355233 @default.
- W2034116338 hasConceptScore W2034116338C55493867 @default.
- W2034116338 hasConceptScore W2034116338C7070889 @default.
- W2034116338 hasConceptScore W2034116338C81885089 @default.
- W2034116338 hasConceptScore W2034116338C86803240 @default.
- W2034116338 hasConceptScore W2034116338C98274493 @default.
- W2034116338 hasIssue "23" @default.
- W2034116338 hasLocation W20341163381 @default.
- W2034116338 hasLocation W20341163382 @default.
- W2034116338 hasOpenAccess W2034116338 @default.
- W2034116338 hasPrimaryLocation W20341163381 @default.
- W2034116338 hasRelatedWork W1966632418 @default.
- W2034116338 hasRelatedWork W2000334988 @default.
- W2034116338 hasRelatedWork W2018786770 @default.
- W2034116338 hasRelatedWork W2027568211 @default.
- W2034116338 hasRelatedWork W2057695275 @default.
- W2034116338 hasRelatedWork W2073112531 @default.
- W2034116338 hasRelatedWork W2105076124 @default.
- W2034116338 hasRelatedWork W2348599162 @default.
- W2034116338 hasRelatedWork W2413532914 @default.
- W2034116338 hasRelatedWork W1969369344 @default.
- W2034116338 hasVolume "65" @default.
- W2034116338 isParatext "false" @default.
- W2034116338 isRetracted "false" @default.
- W2034116338 magId "2034116338" @default.
- W2034116338 workType "article" @default.