FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034117547> ?p ?o ?g. }

• W2034117547 endingPage "17962" @default.
• W2034117547 startingPage "17954" @default.
• W2034117547 abstract "We have designed molecules that permit the selective cross-linking (S-CROSS) of interacting proteins in cell lysates and the sensitive detection of the trapped complexes through in-gel fluorescence scanning. S-CROSS requires the expression of the putative interacting proteins as fusion to CLIP-tag or SNAP-tag, two protein tags that can be specifically labeled with synthetic probes. Bifunctional molecules that contain the substrates of the two tags connected via a fluorophore are used to selectively cross-link interacting proteins in cell lysate. The amount of trapped complex can be then quantified after SDS gel electrophoresis by in-gel fluorescence scanning. On the basis of a detailed kinetic analysis of the cross-linking reaction, we showed that the cross-linking efficiency can be used as an indicator of interaction between two proteins, allowing thereby the unambiguous identification of interacting protein pairs. We validated our approach by confirming a number of interactions through selective cross-linking and showed that it permits the quantitative and simultaneous analysis of multiple homotypic and heterotypic protein complexes and the differentiation between strong and weak protein-protein interactions." @default.
• W2034117547 created "2016-06-24" @default.
• W2034117547 creator A5012515260 @default.
• W2034117547 creator A5026565504 @default.
• W2034117547 creator A5051667493 @default.
• W2034117547 creator A5074868501 @default.
• W2034117547 creator A5078956880 @default.
• W2034117547 date "2009-11-16" @default.
• W2034117547 modified "2023-10-14" @default.
• W2034117547 title "Selective Cross-Linking of Interacting Proteins Using Self-Labeling Tags" @default.
• W2034117547 cites W130088531 @default.
• W2034117547 cites W1984789406 @default.
• W2034117547 cites W1987005832 @default.
• W2034117547 cites W1988464139 @default.
• W2034117547 cites W1994008669 @default.
• W2034117547 cites W1994803330 @default.
• W2034117547 cites W1996160970 @default.
• W2034117547 cites W2001366878 @default.
• W2034117547 cites W2004120029 @default.
• W2034117547 cites W2004605691 @default.
• W2034117547 cites W2010366429 @default.
• W2034117547 cites W2016036293 @default.
• W2034117547 cites W2024377156 @default.
• W2034117547 cites W2026464022 @default.
• W2034117547 cites W2027045807 @default.
• W2034117547 cites W2033735656 @default.
• W2034117547 cites W2050568471 @default.
• W2034117547 cites W2053807240 @default.
• W2034117547 cites W2057141116 @default.
• W2034117547 cites W2064802374 @default.
• W2034117547 cites W2077550665 @default.
• W2034117547 cites W2082359973 @default.
• W2034117547 cites W2095386748 @default.
• W2034117547 cites W2097933346 @default.
• W2034117547 cites W2109261059 @default.
• W2034117547 cites W2110221241 @default.
• W2034117547 cites W2123242499 @default.
• W2034117547 cites W2128036243 @default.
• W2034117547 cites W2128900650 @default.
• W2034117547 cites W2146189740 @default.
• W2034117547 cites W2148304657 @default.
• W2034117547 cites W2149076865 @default.
• W2034117547 cites W2150028503 @default.
• W2034117547 cites W2151721001 @default.
• W2034117547 cites W2166269098 @default.
• W2034117547 cites W2170834903 @default.
• W2034117547 cites W2171935374 @default.
• W2034117547 doi "https://doi.org/10.1021/ja907818q" @default.
• W2034117547 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19916541" @default.
• W2034117547 hasPublicationYear "2009" @default.
• W2034117547 type Work @default.
• W2034117547 sameAs 2034117547 @default.
• W2034117547 citedByCount "59" @default.
• W2034117547 countsByYear W20341175472012 @default.
• W2034117547 countsByYear W20341175472013 @default.
• W2034117547 countsByYear W20341175472014 @default.
• W2034117547 countsByYear W20341175472015 @default.
• W2034117547 countsByYear W20341175472017 @default.
• W2034117547 countsByYear W20341175472018 @default.
• W2034117547 countsByYear W20341175472019 @default.
• W2034117547 countsByYear W20341175472020 @default.
• W2034117547 countsByYear W20341175472021 @default.
• W2034117547 countsByYear W20341175472022 @default.
• W2034117547 countsByYear W20341175472023 @default.
• W2034117547 crossrefType "journal-article" @default.
• W2034117547 hasAuthorship W2034117547A5012515260 @default.
• W2034117547 hasAuthorship W2034117547A5026565504 @default.
• W2034117547 hasAuthorship W2034117547A5051667493 @default.
• W2034117547 hasAuthorship W2034117547A5074868501 @default.
• W2034117547 hasAuthorship W2034117547A5078956880 @default.
• W2034117547 hasConcept C104317684 @default.
• W2034117547 hasConcept C116084860 @default.
• W2034117547 hasConcept C11804247 @default.
• W2034117547 hasConcept C121332964 @default.
• W2034117547 hasConcept C123894998 @default.
• W2034117547 hasConcept C12554922 @default.
• W2034117547 hasConcept C161790260 @default.
• W2034117547 hasConcept C178790620 @default.
• W2034117547 hasConcept C185592680 @default.
• W2034117547 hasConcept C2778617687 @default.
• W2034117547 hasConcept C2780443040 @default.
• W2034117547 hasConcept C32909587 @default.
• W2034117547 hasConcept C40767141 @default.
• W2034117547 hasConcept C55493867 @default.
• W2034117547 hasConcept C57409179 @default.
• W2034117547 hasConcept C62520636 @default.
• W2034117547 hasConcept C86803240 @default.
• W2034117547 hasConcept C91881484 @default.
• W2034117547 hasConceptScore W2034117547C104317684 @default.
• W2034117547 hasConceptScore W2034117547C116084860 @default.
• W2034117547 hasConceptScore W2034117547C11804247 @default.
• W2034117547 hasConceptScore W2034117547C121332964 @default.
• W2034117547 hasConceptScore W2034117547C123894998 @default.
• W2034117547 hasConceptScore W2034117547C12554922 @default.
• W2034117547 hasConceptScore W2034117547C161790260 @default.
• W2034117547 hasConceptScore W2034117547C178790620 @default.
• W2034117547 hasConceptScore W2034117547C185592680 @default.
• W2034117547 hasConceptScore W2034117547C2778617687 @default.

• next