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- W2034117918 abstract "Understanding the process of ex vivo embryonic stem (ES) cell differentiation is important for generating higher yields of desirable cell types or lineages and for understanding fundamental aspects of ES differentiation. We used DNA microarray analysis to investigate the differentiation of mouse ES cells cultured under three differentiation conditions. Embryoid body (EB) formation was compared to differentiation on surfaces coated with either gelatin (GEL) or matrigel (MAT). Based on the transcriptional patterns of a list of literature-based “stemness” genes, ES cell differentiation on the two coated surfaces appeared similar but not identical to EB differentiation. A notable difference was the GEL and MAT upregulation but EB downregulation of nine such stemness genes, which are related to cell adhesion and epithelial differentiation. Further, GEL and MAT differentiation showed higher expression of bone formation–related genes (Spp1, Csf1, Gsn, Bmp8b, Crlf1). Gene ontology analysis shows an increase in the expression of genes related to migration and cell structure in all three conditions. Overall, GEL and MAT conditions resulted in a more similar to each other transcriptional profile than to the EB condition, and such differences are apparently related to higher nutrient and metabolite gradients and limitations in the EB versus the GEL or MAT cultures." @default.
- W2034117918 created "2016-06-24" @default.
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- W2034117918 date "2008-10-01" @default.
- W2034117918 modified "2023-10-02" @default.
- W2034117918 title "Comparative Transcriptional Analysis of Embryoid Body Versus Two-Dimensional Differentiation of Murine Embryonic Stem Cells" @default.
- W2034117918 cites W1520384293 @default.
- W2034117918 cites W1524805223 @default.
- W2034117918 cites W1552674655 @default.
- W2034117918 cites W1587777046 @default.
- W2034117918 cites W1608762409 @default.
- W2034117918 cites W1639816383 @default.
- W2034117918 cites W165273793 @default.
- W2034117918 cites W1811045671 @default.
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- W2034117918 cites W1966697925 @default.
- W2034117918 cites W1972343772 @default.
- W2034117918 cites W1978099800 @default.
- W2034117918 cites W1983274440 @default.
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- W2034117918 cites W1988296009 @default.
- W2034117918 cites W1991681682 @default.
- W2034117918 cites W1995168697 @default.
- W2034117918 cites W2000320999 @default.
- W2034117918 cites W2001627351 @default.
- W2034117918 cites W2003240618 @default.
- W2034117918 cites W2005505016 @default.
- W2034117918 cites W2009434462 @default.
- W2034117918 cites W2011159069 @default.
- W2034117918 cites W2011181507 @default.
- W2034117918 cites W2014073178 @default.
- W2034117918 cites W2016533426 @default.
- W2034117918 cites W2019798761 @default.
- W2034117918 cites W2019813306 @default.
- W2034117918 cites W2020915199 @default.
- W2034117918 cites W2025739925 @default.
- W2034117918 cites W2027630408 @default.
- W2034117918 cites W2032711435 @default.
- W2034117918 cites W2036924945 @default.
- W2034117918 cites W2037795517 @default.
- W2034117918 cites W2040613244 @default.
- W2034117918 cites W2045388302 @default.
- W2034117918 cites W2045459881 @default.
- W2034117918 cites W2053777072 @default.
- W2034117918 cites W2057570836 @default.
- W2034117918 cites W2060926602 @default.
- W2034117918 cites W2063822655 @default.
- W2034117918 cites W2066673889 @default.
- W2034117918 cites W2066960006 @default.
- W2034117918 cites W2076770967 @default.
- W2034117918 cites W2077779082 @default.
- W2034117918 cites W2082305141 @default.
- W2034117918 cites W2088016889 @default.
- W2034117918 cites W2100012145 @default.
- W2034117918 cites W2101840599 @default.
- W2034117918 cites W2104269891 @default.
- W2034117918 cites W2104878392 @default.
- W2034117918 cites W2107658299 @default.
- W2034117918 cites W2110097720 @default.
- W2034117918 cites W2111790269 @default.
- W2034117918 cites W2115226431 @default.
- W2034117918 cites W2119705791 @default.
- W2034117918 cites W2119739306 @default.
- W2034117918 cites W2119958096 @default.
- W2034117918 cites W2122683221 @default.
- W2034117918 cites W2123375975 @default.
- W2034117918 cites W2127180583 @default.
- W2034117918 cites W2127432537 @default.
- W2034117918 cites W2130728804 @default.
- W2034117918 cites W2135210803 @default.
- W2034117918 cites W2137280750 @default.
- W2034117918 cites W2137540340 @default.
- W2034117918 cites W2138778307 @default.
- W2034117918 cites W2139961479 @default.
- W2034117918 cites W2142672099 @default.
- W2034117918 cites W2149922101 @default.
- W2034117918 cites W2150094514 @default.
- W2034117918 cites W2150926065 @default.
- W2034117918 cites W2151322467 @default.
- W2034117918 cites W2152169882 @default.
- W2034117918 cites W2154379321 @default.
- W2034117918 cites W2154484742 @default.
- W2034117918 cites W2155062057 @default.
- W2034117918 cites W2165588237 @default.
- W2034117918 cites W2166870017 @default.
- W2034117918 cites W2173863177 @default.
- W2034117918 cites W2183826319 @default.
- W2034117918 cites W2318784916 @default.
- W2034117918 cites W45534775 @default.
- W2034117918 doi "https://doi.org/10.1089/ten.tea.2007.0331" @default.
- W2034117918 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18433312" @default.
- W2034117918 hasPublicationYear "2008" @default.
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