Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034119102> ?p ?o ?g. }
- W2034119102 endingPage "719" @default.
- W2034119102 startingPage "707" @default.
- W2034119102 abstract "The proteolytic enzyme trypsin was chemically immobilized to an amine-functionalized sol-gel using adipoyl chloride under nonaqueous conditions and a nitrogen atmosphere. In the synthesis of the sol-gel, tetraethyl orthosilicate (TEOS), and 3-(2-aminoethylamino) propyldimethoxymethylsilane (AEAPMS) (50:50, v/v) were used, which provided convenient physical and chemical conditions to maintain catalytic activity of immobilized trypsin molecules for the digestion of proteins in proteomics applications. Bovine serum albumin was used as a model protein to perform enzymatic digestion using the trypsin immobilized sol-gel. The resulting peptides were analyzed by matrix-assisted laser desorption/ionization-mass spectrometry to evaluate the digestion performance and specificity of the sol-gel material. The trypsin immobilized sol-gel showed superior enzymatic activity in protein digestion and it was determined that the sol-gel material could be repeatedly used at least 25 times without significant activity loss in long-term use. Additionally, autocatalysis was prevented by immobilization of trypsin. The peptide digest having the highest purity was obtained for protein identification studies." @default.
- W2034119102 created "2016-06-24" @default.
- W2034119102 creator A5007175913 @default.
- W2034119102 creator A5049399473 @default.
- W2034119102 creator A5073543970 @default.
- W2034119102 creator A5075051141 @default.
- W2034119102 date "2014-03-03" @default.
- W2034119102 modified "2023-09-24" @default.
- W2034119102 title "A Trypsin Immobilized Sol-Gel for Protein Indentification in MALDI-MS Applications" @default.
- W2034119102 cites W118777696 @default.
- W2034119102 cites W1518520618 @default.
- W2034119102 cites W1525575010 @default.
- W2034119102 cites W1964760292 @default.
- W2034119102 cites W1970574159 @default.
- W2034119102 cites W1989265845 @default.
- W2034119102 cites W1994899469 @default.
- W2034119102 cites W1995297318 @default.
- W2034119102 cites W2003261719 @default.
- W2034119102 cites W2005191262 @default.
- W2034119102 cites W2007463705 @default.
- W2034119102 cites W2012484827 @default.
- W2034119102 cites W2016564563 @default.
- W2034119102 cites W2024078329 @default.
- W2034119102 cites W2025776351 @default.
- W2034119102 cites W2032716518 @default.
- W2034119102 cites W2039591679 @default.
- W2034119102 cites W2041993377 @default.
- W2034119102 cites W2042748079 @default.
- W2034119102 cites W2053766086 @default.
- W2034119102 cites W2054016284 @default.
- W2034119102 cites W2057493696 @default.
- W2034119102 cites W2059737503 @default.
- W2034119102 cites W2061007134 @default.
- W2034119102 cites W2067859832 @default.
- W2034119102 cites W2070282268 @default.
- W2034119102 cites W2089441741 @default.
- W2034119102 cites W2093712513 @default.
- W2034119102 cites W2129485427 @default.
- W2034119102 cites W2140985419 @default.
- W2034119102 cites W2143993735 @default.
- W2034119102 cites W2147144294 @default.
- W2034119102 cites W2157816048 @default.
- W2034119102 cites W2171938017 @default.
- W2034119102 cites W2323472998 @default.
- W2034119102 cites W4241765787 @default.
- W2034119102 doi "https://doi.org/10.1080/00032719.2013.831423" @default.
- W2034119102 hasPublicationYear "2014" @default.
- W2034119102 type Work @default.
- W2034119102 sameAs 2034119102 @default.
- W2034119102 citedByCount "4" @default.
- W2034119102 countsByYear W20341191022014 @default.
- W2034119102 countsByYear W20341191022015 @default.
- W2034119102 countsByYear W20341191022017 @default.
- W2034119102 crossrefType "journal-article" @default.
- W2034119102 hasAuthorship W2034119102A5007175913 @default.
- W2034119102 hasAuthorship W2034119102A5049399473 @default.
- W2034119102 hasAuthorship W2034119102A5073543970 @default.
- W2034119102 hasAuthorship W2034119102A5075051141 @default.
- W2034119102 hasConcept C150394285 @default.
- W2034119102 hasConcept C162356407 @default.
- W2034119102 hasConcept C162711632 @default.
- W2034119102 hasConcept C178790620 @default.
- W2034119102 hasConcept C181199279 @default.
- W2034119102 hasConcept C185592680 @default.
- W2034119102 hasConcept C2780340462 @default.
- W2034119102 hasConcept C43617362 @default.
- W2034119102 hasConcept C55493867 @default.
- W2034119102 hasConcept C75280812 @default.
- W2034119102 hasConcept C86807702 @default.
- W2034119102 hasConceptScore W2034119102C150394285 @default.
- W2034119102 hasConceptScore W2034119102C162356407 @default.
- W2034119102 hasConceptScore W2034119102C162711632 @default.
- W2034119102 hasConceptScore W2034119102C178790620 @default.
- W2034119102 hasConceptScore W2034119102C181199279 @default.
- W2034119102 hasConceptScore W2034119102C185592680 @default.
- W2034119102 hasConceptScore W2034119102C2780340462 @default.
- W2034119102 hasConceptScore W2034119102C43617362 @default.
- W2034119102 hasConceptScore W2034119102C55493867 @default.
- W2034119102 hasConceptScore W2034119102C75280812 @default.
- W2034119102 hasConceptScore W2034119102C86807702 @default.
- W2034119102 hasIssue "4" @default.
- W2034119102 hasLocation W20341191021 @default.
- W2034119102 hasOpenAccess W2034119102 @default.
- W2034119102 hasPrimaryLocation W20341191021 @default.
- W2034119102 hasRelatedWork W1965143182 @default.
- W2034119102 hasRelatedWork W1994987472 @default.
- W2034119102 hasRelatedWork W2029952678 @default.
- W2034119102 hasRelatedWork W2031508767 @default.
- W2034119102 hasRelatedWork W2046532202 @default.
- W2034119102 hasRelatedWork W2050299159 @default.
- W2034119102 hasRelatedWork W2054156647 @default.
- W2034119102 hasRelatedWork W2529484206 @default.
- W2034119102 hasRelatedWork W2773553194 @default.
- W2034119102 hasRelatedWork W3141521293 @default.
- W2034119102 hasVolume "47" @default.
- W2034119102 isParatext "false" @default.
- W2034119102 isRetracted "false" @default.
- W2034119102 magId "2034119102" @default.