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- W2034125126 abstract "A selective and sensitive liquid chromatographic/tandem mass spectrometric method was developed and validated for the matrix effect controlled estimation of sildenafil and N-desmethyl sildenafil in human plasma. The samples were extracted on solid-phase extraction cartridges and chromatographed on XTerra MS C18 5 µm (4.6 mm x 150 mm) column using isocratic mobile phase. The method was validated over the concentration range of 5.00–1401.35 ng/mL for sildenafil and 2.83–603.38 ng/mL for N-desmethyl sildenafil. The parent to product ion transitions for sildenafil, N-desmethyl sildenafil and quetiapine were m/z 475.10–283.30; 461.20–283.20 and 384.20–253.20, respectively. Matrix factor was assessed using post-extraction addition technique and found to be controlled. Matrix effect was assessed using post-column infusion technique and the co-eluting matrix components found to be chromatographically separated. The analytes were stable in standard solutions and in plasma samples under typical storage and processi..." @default.
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- W2034125126 date "2013-01-02" @default.
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- W2034125126 title "DEVELOPMENT AND VALIDATION OF BIOANALYTICAL METHOD FOR MATRIX EFFECT CONTROLLED ESTIMATION OF SILDENAFIL AND N-DESMETHYL SILDENAFIL IN HUMAN PLASMA USING LC-MS/MS" @default.
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