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• W2034130780 startingPage "1359" @default.
• W2034130780 abstract "As part of the WHO Network for HIV Isolation and Characterization, we PCR amplified, cloned, and sequenced gp120 and gp160 genes from 12 HIV-1 isolates collected in four WHO-sponsored vaccine evaluation sites (Brazil, Rwanda, Thailand, Uganda). Envelope clones were derived from PBMC-grown isolates obtained from asymptomatic individuals within 2 years of seroconversion. Analysis of their deduced amino acid sequences identified all but one to contain an uninterrupted open reading frame. Transient expression and biological characterization of selected gp160 constructs identified six clones to encode full length and functional envelope glycoproteins. Phylogenetic analysis of their nucleotide sequences revealed that they represent HIV-1 subtypes A, B, C, and E. Since current knowledge of HIV-1 envelope immunobiology is almost exclusively derived from subtype B viruses, these reagents should facilitate future envelope structure, function and antigenicity studies on a broader spectrum of viruses. This should assist in the design and evaluation of effective vaccines against HIV-1." @default.
• W2034130780 created "2016-06-24" @default.
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• W2034130780 date "1994-11-01" @default.
• W2034130780 modified "2023-10-14" @default.
• W2034130780 title "Genetic Variation of HIV Type 1 in Four World Health Organization-Sponsored Vaccine Evaluation Sites: Generation of Functional Envelope (Glycoprotein 160) Clones Representative of Sequence Subtypes A, B, C, and E" @default.
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• W2034130780 doi "https://doi.org/10.1089/aid.1994.10.1359" @default.
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