FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034137085> ?p ?o ?g. }

• W2034137085 endingPage "1132" @default.
• W2034137085 startingPage "1125" @default.
• W2034137085 abstract "Isolated macronuclei from the hypotrichous ciliated protozoan Euplotes eurystomus incorporate biotinylated dUTP specifically into the replication band (RB) as detected with immunofluorescence, using rabbit anti-biotin antibodies followed by fluorescein-conjugated goat anti-rabbit IgG. When gold-conjugated goat anti-rabbit IgG was used in a preembedded reaction, subsequent immunoelectron microscopic analysis demonstrated that the biotinylated nucleotide appeared more concentrated in the rear zone of the RB, with almost no labeling in the forward zone. It was possible to use the immunofluorescent assay to establish that incorporation of biotinylated dUTP is inhibited by simultaneous addition of N-ethyl maleimide or aphidicolin, and by omission of any one of the other unlabeled dNTPs. In addition, prolonged heat shock of the intact cells, before lysis and in vitro assay, yielded markedly reduced incorporation. Comparison with published data on the in vivo incorporation of [3H]thymidine into Euplotes eurystomus RBs indicates the fidelity of the in vitro reaction." @default.
• W2034137085 created "2016-06-24" @default.
• W2034137085 creator A5015247015 @default.
• W2034137085 creator A5080496407 @default.
• W2034137085 date "1987-05-01" @default.
• W2034137085 modified "2023-09-26" @default.
• W2034137085 title "In vitro DNA synthesis in the macronuclear replication band of Euplotes eurystomus." @default.
• W2034137085 cites W130152560 @default.
• W2034137085 cites W1457627147 @default.
• W2034137085 cites W1508775395 @default.
• W2034137085 cites W1601085105 @default.
• W2034137085 cites W1671970356 @default.
• W2034137085 cites W1967710870 @default.
• W2034137085 cites W1971240230 @default.
• W2034137085 cites W1985472433 @default.
• W2034137085 cites W1991446406 @default.
• W2034137085 cites W1994625971 @default.
• W2034137085 cites W1996382155 @default.
• W2034137085 cites W2006450491 @default.
• W2034137085 cites W2012233564 @default.
• W2034137085 cites W2014038908 @default.
• W2034137085 cites W2015956661 @default.
• W2034137085 cites W2020152761 @default.
• W2034137085 cites W2031468048 @default.
• W2034137085 cites W2034017431 @default.
• W2034137085 cites W2047927121 @default.
• W2034137085 cites W2053997765 @default.
• W2034137085 cites W2083600877 @default.
• W2034137085 cites W2085004527 @default.
• W2034137085 cites W2087881172 @default.
• W2034137085 cites W2104855244 @default.
• W2034137085 cites W2115494657 @default.
• W2034137085 cites W2140243527 @default.
• W2034137085 cites W2147274177 @default.
• W2034137085 cites W2396033980 @default.
• W2034137085 cites W2405063277 @default.
• W2034137085 doi "https://doi.org/10.1083/jcb.104.5.1125" @default.
• W2034137085 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2114460" @default.
• W2034137085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3553204" @default.
• W2034137085 hasPublicationYear "1987" @default.
• W2034137085 type Work @default.
• W2034137085 sameAs 2034137085 @default.
• W2034137085 citedByCount "18" @default.
• W2034137085 crossrefType "journal-article" @default.
• W2034137085 hasAuthorship W2034137085A5015247015 @default.
• W2034137085 hasAuthorship W2034137085A5080496407 @default.
• W2034137085 hasBestOaLocation W20341370851 @default.
• W2034137085 hasConcept C121332964 @default.
• W2034137085 hasConcept C150903083 @default.
• W2034137085 hasConcept C153911025 @default.
• W2034137085 hasConcept C159654299 @default.
• W2034137085 hasConcept C202751555 @default.
• W2034137085 hasConcept C203014093 @default.
• W2034137085 hasConcept C204628709 @default.
• W2034137085 hasConcept C207001950 @default.
• W2034137085 hasConcept C2776253266 @default.
• W2034137085 hasConcept C2778015030 @default.
• W2034137085 hasConcept C2778204606 @default.
• W2034137085 hasConcept C2778236600 @default.
• W2034137085 hasConcept C2993140218 @default.
• W2034137085 hasConcept C552990157 @default.
• W2034137085 hasConcept C55493867 @default.
• W2034137085 hasConcept C62520636 @default.
• W2034137085 hasConcept C64241487 @default.
• W2034137085 hasConcept C86803240 @default.
• W2034137085 hasConcept C91881484 @default.
• W2034137085 hasConceptScore W2034137085C121332964 @default.
• W2034137085 hasConceptScore W2034137085C150903083 @default.
• W2034137085 hasConceptScore W2034137085C153911025 @default.
• W2034137085 hasConceptScore W2034137085C159654299 @default.
• W2034137085 hasConceptScore W2034137085C202751555 @default.
• W2034137085 hasConceptScore W2034137085C203014093 @default.
• W2034137085 hasConceptScore W2034137085C204628709 @default.
• W2034137085 hasConceptScore W2034137085C207001950 @default.
• W2034137085 hasConceptScore W2034137085C2776253266 @default.
• W2034137085 hasConceptScore W2034137085C2778015030 @default.
• W2034137085 hasConceptScore W2034137085C2778204606 @default.
• W2034137085 hasConceptScore W2034137085C2778236600 @default.
• W2034137085 hasConceptScore W2034137085C2993140218 @default.
• W2034137085 hasConceptScore W2034137085C552990157 @default.
• W2034137085 hasConceptScore W2034137085C55493867 @default.
• W2034137085 hasConceptScore W2034137085C62520636 @default.
• W2034137085 hasConceptScore W2034137085C64241487 @default.
• W2034137085 hasConceptScore W2034137085C86803240 @default.
• W2034137085 hasConceptScore W2034137085C91881484 @default.
• W2034137085 hasIssue "5" @default.
• W2034137085 hasLocation W20341370851 @default.
• W2034137085 hasLocation W20341370852 @default.
• W2034137085 hasLocation W20341370853 @default.
• W2034137085 hasLocation W20341370854 @default.
• W2034137085 hasOpenAccess W2034137085 @default.
• W2034137085 hasPrimaryLocation W20341370851 @default.
• W2034137085 hasRelatedWork W1892522246 @default.
• W2034137085 hasRelatedWork W1927061416 @default.
• W2034137085 hasRelatedWork W2002473487 @default.
• W2034137085 hasRelatedWork W2006711628 @default.
• W2034137085 hasRelatedWork W2032116141 @default.
• W2034137085 hasRelatedWork W2034137085 @default.

• next