FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034139627> ?p ?o ?g. }

• W2034139627 endingPage "863" @default.
• W2034139627 startingPage "843" @default.
• W2034139627 abstract "Human plasma proteins were separated by 2-DE under nondenaturing conditions followed by the assignment of the CBB-stained spots using MALDI-MS and PMF, aiming to correlate the information of intact proteins with that of constituent polypeptides. A microgel system was employed to facilitate the analysis. Totally 157 spots on a nondenaturing micro-2-DE gel were numbered, the spots were excised, the proteins in the gel pieces were subjected to in-gel digestion with trypsin followed by polypeptide analysis using MALDI-MS and PMF. Two PMF algorithms, MASCOT (with Swiss-Prot database) and ProFound (with NCBInr database) were employed. A total of 153 spots out of the 157 provided significant match (p <0.05) with polypeptides in databases. Eighty spots were assigned to contain multiple (2–4) polypeptides, suggesting (i) noncovalent interaction between proteins/polypeptides, (ii) disulfide bonding of polypeptides, or (iii) overlapping of the protein locations on the gel. The results of polypeptide assignment coincided very well with the results of protein mapping previously reported, in which 33 plasma proteins were identified using blotting-immunochemical staining (Manabe, T., Takahashi, Y., Higuchi, N., Okuyama, T., Electrophoresis 1985, 6, 462–467). Further, 19 polypeptides in 25 spots were newly assigned. These results demonstrate that the techniques of MALDI-MS and PMF can be applied for analysis of proteins separated on nondenaturing 2-DE gels, providing information on their polypeptide structure. The integrated information on proteins and polypeptides would help the comprehensive understanding on the functions of complex protein systems." @default.
• W2034139627 created "2016-06-24" @default.
• W2034139627 creator A5053332830 @default.
• W2034139627 creator A5078917552 @default.
• W2034139627 creator A5079667330 @default.
• W2034139627 date "2007-03-01" @default.
• W2034139627 modified "2023-10-06" @default.
• W2034139627 title "Assignment of human plasma polypeptides on a nondenaturing 2-D gel using MALDI-MS and PMF and comparisons with the results of intact protein mapping" @default.
• W2034139627 cites W1603545969 @default.
• W2034139627 cites W1756973693 @default.
• W2034139627 cites W1963530308 @default.
• W2034139627 cites W1965383843 @default.
• W2034139627 cites W1966809971 @default.
• W2034139627 cites W1968056160 @default.
• W2034139627 cites W1974389262 @default.
• W2034139627 cites W1981593008 @default.
• W2034139627 cites W1985560593 @default.
• W2034139627 cites W2002396315 @default.
• W2034139627 cites W2031001696 @default.
• W2034139627 cites W2035998381 @default.
• W2034139627 cites W2045144962 @default.
• W2034139627 cites W2055063265 @default.
• W2034139627 cites W2058128222 @default.
• W2034139627 cites W2060219525 @default.
• W2034139627 cites W2060326669 @default.
• W2034139627 cites W2064323228 @default.
• W2034139627 cites W2078594804 @default.
• W2034139627 cites W2081249438 @default.
• W2034139627 cites W2085411110 @default.
• W2034139627 cites W2086680947 @default.
• W2034139627 cites W2087746647 @default.
• W2034139627 cites W2092523771 @default.
• W2034139627 cites W2113874481 @default.
• W2034139627 cites W2120340740 @default.
• W2034139627 cites W2144468049 @default.
• W2034139627 cites W2154798437 @default.
• W2034139627 cites W4301259840 @default.
• W2034139627 doi "https://doi.org/10.1002/elps.200600389" @default.
• W2034139627 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17274101" @default.
• W2034139627 hasPublicationYear "2007" @default.
• W2034139627 type Work @default.
• W2034139627 sameAs 2034139627 @default.
• W2034139627 citedByCount "34" @default.
• W2034139627 countsByYear W20341396272012 @default.
• W2034139627 countsByYear W20341396272013 @default.
• W2034139627 countsByYear W20341396272014 @default.
• W2034139627 countsByYear W20341396272015 @default.
• W2034139627 countsByYear W20341396272016 @default.
• W2034139627 countsByYear W20341396272017 @default.
• W2034139627 countsByYear W20341396272019 @default.
• W2034139627 countsByYear W20341396272020 @default.
• W2034139627 countsByYear W20341396272021 @default.
• W2034139627 crossrefType "journal-article" @default.
• W2034139627 hasAuthorship W2034139627A5053332830 @default.
• W2034139627 hasAuthorship W2034139627A5078917552 @default.
• W2034139627 hasAuthorship W2034139627A5079667330 @default.
• W2034139627 hasConcept C101660505 @default.
• W2034139627 hasConcept C104317684 @default.
• W2034139627 hasConcept C116084860 @default.
• W2034139627 hasConcept C147789679 @default.
• W2034139627 hasConcept C153911025 @default.
• W2034139627 hasConcept C181199279 @default.
• W2034139627 hasConcept C185592680 @default.
• W2034139627 hasConcept C2780340462 @default.
• W2034139627 hasConcept C2780761556 @default.
• W2034139627 hasConcept C2781255879 @default.
• W2034139627 hasConcept C40250595 @default.
• W2034139627 hasConcept C43617362 @default.
• W2034139627 hasConcept C46111723 @default.
• W2034139627 hasConcept C47793336 @default.
• W2034139627 hasConcept C54355233 @default.
• W2034139627 hasConcept C55493867 @default.
• W2034139627 hasConcept C74864618 @default.
• W2034139627 hasConcept C86803240 @default.
• W2034139627 hasConceptScore W2034139627C101660505 @default.
• W2034139627 hasConceptScore W2034139627C104317684 @default.
• W2034139627 hasConceptScore W2034139627C116084860 @default.
• W2034139627 hasConceptScore W2034139627C147789679 @default.
• W2034139627 hasConceptScore W2034139627C153911025 @default.
• W2034139627 hasConceptScore W2034139627C181199279 @default.
• W2034139627 hasConceptScore W2034139627C185592680 @default.
• W2034139627 hasConceptScore W2034139627C2780340462 @default.
• W2034139627 hasConceptScore W2034139627C2780761556 @default.
• W2034139627 hasConceptScore W2034139627C2781255879 @default.
• W2034139627 hasConceptScore W2034139627C40250595 @default.
• W2034139627 hasConceptScore W2034139627C43617362 @default.
• W2034139627 hasConceptScore W2034139627C46111723 @default.
• W2034139627 hasConceptScore W2034139627C47793336 @default.
• W2034139627 hasConceptScore W2034139627C54355233 @default.
• W2034139627 hasConceptScore W2034139627C55493867 @default.
• W2034139627 hasConceptScore W2034139627C74864618 @default.
• W2034139627 hasConceptScore W2034139627C86803240 @default.
• W2034139627 hasIssue "5" @default.
• W2034139627 hasLocation W20341396271 @default.
• W2034139627 hasLocation W20341396272 @default.
• W2034139627 hasOpenAccess W2034139627 @default.
• W2034139627 hasPrimaryLocation W20341396271 @default.
• W2034139627 hasRelatedWork W1759726671 @default.

• next