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- W2034140183 abstract "A simple method for the measurement of estradiol-17β, estrone, testosterone and androstenedione in 4–6 ml of human male and female plasma is described. By ether extraction of alkalinized plasma followed by a single celite column Chromatographic step or by two column Chromatographic steps on Sephadex LH 20, purified extracts are obtained for competitive protein binding radioassay. Cytosol from six-day pregnant rabbit uteri (according to Korenman) was used for estrogen assays, and human plasma from the third trimester of pregnancy (according to Sciarra) was used for androgen assays. The plasma values of testosterone and androstenedione were comparable with those obtained after t.l.c. and competitive protein binding. The specificity and sensitivity of the method enabled determinations of plasma estrogen to be made not only in normal adults, but also in hypogonadal patients where estradiol-17β values are at very low levels. The accuracy of these determinations was confirmed by repeated measurements of blanks which range from 0 to 5 pg/ml, and by recovery experiments of known amounts of added estradiol-17β." @default.
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- W2034140183 date "1972-06-01" @default.
- W2034140183 modified "2023-09-25" @default.
- W2034140183 title "A simple procedure for the combined determination of plasma estrogen and androgen concentrations by competitive protein binding analysis" @default.
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- W2034140183 doi "https://doi.org/10.1016/0022-4731(72)90064-7" @default.
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