FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034142671> ?p ?o ?g. }

• W2034142671 endingPage "26" @default.
• W2034142671 startingPage "19" @default.
• W2034142671 abstract "This study shows the thermo-stability of lyophilized and purified recombinant VP7 bluetongue virus (BTV) protein in the presence of two sugar stabilizers (trehalose and mannitol) at different temperature. Truncated VP7 protein purified by nickel affinity column was lyophilized in the presence of trehalose and mannitol at 60 mM final concentration and then exposed to different temperature like 4, 25, 37 and 45 °C for various periods like 5 months, 7 weeks, 7 days and 48 h, respectively. After thermal treatment, the reactivity of the protein was evaluated in indirect ELISA. At 4 and 25 °C, the protein was stable up to 5 months and 7 weeks, respectively, irrespective of stabilizers used. At 37 °C, it was stable up to 3 days with both the stabilizers, after which it lost its stability and reactivity. At 45 °C, the protein was stable up to 30 and 24 h with trehalose and mannitol stabilizers, respectively. Both stabilizers found suitable for stability of the protein. However, trehalose appeared to have better stabilizing effect, particularly at higher temperatures than the mannitol. Trehalose could be used as stabilizer for freeze-drying the recombinant VP7 protein if an indirect ELISA kit based on the purified rVP7 protein is supplied to different laboratories of the country for detection of BTV antibody in sheep." @default.
• W2034142671 created "2016-06-24" @default.
• W2034142671 creator A5020581174 @default.
• W2034142671 creator A5023859199 @default.
• W2034142671 creator A5039358684 @default.
• W2034142671 creator A5054758162 @default.
• W2034142671 creator A5069080399 @default.
• W2034142671 date "2015-02-10" @default.
• W2034142671 modified "2023-09-25" @default.
• W2034142671 title "Evaluation of thermo-stability of bluetongue virus recombinant VP7 antigen in indirect ELISA" @default.
• W2034142671 cites W1493124133 @default.
• W2034142671 cites W1602974455 @default.
• W2034142671 cites W1613350179 @default.
• W2034142671 cites W1975502171 @default.
• W2034142671 cites W1975681078 @default.
• W2034142671 cites W1976810555 @default.
• W2034142671 cites W1985436384 @default.
• W2034142671 cites W1987451065 @default.
• W2034142671 cites W1992106431 @default.
• W2034142671 cites W1993054679 @default.
• W2034142671 cites W2017027704 @default.
• W2034142671 cites W2027231861 @default.
• W2034142671 cites W2038975045 @default.
• W2034142671 cites W2039621224 @default.
• W2034142671 cites W2044557357 @default.
• W2034142671 cites W2046331902 @default.
• W2034142671 cites W2050425137 @default.
• W2034142671 cites W2057517669 @default.
• W2034142671 cites W2063728090 @default.
• W2034142671 cites W2071050148 @default.
• W2034142671 cites W2077231187 @default.
• W2034142671 cites W2088169918 @default.
• W2034142671 cites W2092817296 @default.
• W2034142671 cites W2103340838 @default.
• W2034142671 cites W2131692566 @default.
• W2034142671 cites W2133114460 @default.
• W2034142671 cites W2327631505 @default.
• W2034142671 cites W2416112900 @default.
• W2034142671 cites W2416856743 @default.
• W2034142671 cites W2489508617 @default.
• W2034142671 doi "https://doi.org/10.1007/s13337-014-0244-6" @default.
• W2034142671 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4585054" @default.
• W2034142671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26436117" @default.
• W2034142671 hasPublicationYear "2015" @default.
• W2034142671 type Work @default.
• W2034142671 sameAs 2034142671 @default.
• W2034142671 citedByCount "3" @default.
• W2034142671 countsByYear W20341426712019 @default.
• W2034142671 countsByYear W20341426712020 @default.
• W2034142671 countsByYear W20341426712021 @default.
• W2034142671 crossrefType "journal-article" @default.
• W2034142671 hasAuthorship W2034142671A5020581174 @default.
• W2034142671 hasAuthorship W2034142671A5023859199 @default.
• W2034142671 hasAuthorship W2034142671A5039358684 @default.
• W2034142671 hasAuthorship W2034142671A5054758162 @default.
• W2034142671 hasAuthorship W2034142671A5069080399 @default.
• W2034142671 hasBestOaLocation W20341426712 @default.
• W2034142671 hasConcept C104317684 @default.
• W2034142671 hasConcept C127413603 @default.
• W2034142671 hasConcept C147483822 @default.
• W2034142671 hasConcept C178790620 @default.
• W2034142671 hasConcept C185592680 @default.
• W2034142671 hasConcept C203014093 @default.
• W2034142671 hasConcept C2777502316 @default.
• W2034142671 hasConcept C2778445172 @default.
• W2034142671 hasConcept C2778452849 @default.
• W2034142671 hasConcept C40767141 @default.
• W2034142671 hasConcept C43617362 @default.
• W2034142671 hasConcept C55493867 @default.
• W2034142671 hasConcept C59061564 @default.
• W2034142671 hasConcept C78519656 @default.
• W2034142671 hasConcept C86803240 @default.
• W2034142671 hasConceptScore W2034142671C104317684 @default.
• W2034142671 hasConceptScore W2034142671C127413603 @default.
• W2034142671 hasConceptScore W2034142671C147483822 @default.
• W2034142671 hasConceptScore W2034142671C178790620 @default.
• W2034142671 hasConceptScore W2034142671C185592680 @default.
• W2034142671 hasConceptScore W2034142671C203014093 @default.
• W2034142671 hasConceptScore W2034142671C2777502316 @default.
• W2034142671 hasConceptScore W2034142671C2778445172 @default.
• W2034142671 hasConceptScore W2034142671C2778452849 @default.
• W2034142671 hasConceptScore W2034142671C40767141 @default.
• W2034142671 hasConceptScore W2034142671C43617362 @default.
• W2034142671 hasConceptScore W2034142671C55493867 @default.
• W2034142671 hasConceptScore W2034142671C59061564 @default.
• W2034142671 hasConceptScore W2034142671C78519656 @default.
• W2034142671 hasConceptScore W2034142671C86803240 @default.
• W2034142671 hasIssue "1-2" @default.
• W2034142671 hasLocation W20341426711 @default.
• W2034142671 hasLocation W20341426712 @default.
• W2034142671 hasLocation W20341426713 @default.
• W2034142671 hasLocation W20341426714 @default.
• W2034142671 hasOpenAccess W2034142671 @default.
• W2034142671 hasPrimaryLocation W20341426711 @default.
• W2034142671 hasRelatedWork W2006826318 @default.
• W2034142671 hasRelatedWork W2013325378 @default.
• W2034142671 hasRelatedWork W2017027704 @default.
• W2034142671 hasRelatedWork W2064368752 @default.

• next