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• W2034143076 startingPage "491" @default.
• W2034143076 abstract "The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fusion DNA of its 5'-upstream region in front of the gene for firefly luciferase. Using this construct in a transient assay, we determined a pra2 cis-regulatory region sufficient to direct the light down-regulation of the luciferase reporter gene. Both 5'- and internal deletion analyses revealed that the 93-bp sequence between -734 and -642 from the transcriptional start site was important for phytochrome down-regulation. Gain-of-function analysis showed that this 93-bp region could confer light down-regulation when fused to the cauliflower mosaic virus 35S promoter. Furthermore, linker-scanning analysis showed that a 12-bp sequence within the 93-bp region mediated phytochrome down-regulation. Gel-retardation analysis showed the presence of a nuclear factor that was specifically bound to the 12-bp sequence in vitro. These results indicate that this element is a cis-regulatory element involved in phytochrome down-regulated expression." @default.
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• W2034143076 date "1999-06-01" @default.
• W2034143076 modified "2023-10-16" @default.
• W2034143076 title "Identification of a <i>cis</i>-Regulatory Element Involved in Phytochrome Down-Regulated Expression of the Pea Small GTPase Gene <i>pra2</i>1" @default.
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• W2034143076 doi "https://doi.org/10.1104/pp.120.2.491" @default.
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