Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034143658> ?p ?o ?g. }
- W2034143658 abstract "We have identified and characterized a homolog of the 40 kDa cyclophilins in the budding yeast Saccharomyces cerevisiae. At the amino acid level, this novel yeast cyclophilin, termed Cyp40, is 47% identical to human cyclophilin-40. Recombinant Cyp40 produced in bacteria has a peptidyl-prolyl cis-trans isomerase activity with a catalytic efficiency (k[cat]/K[m]) of 0.5 x 10(6)M(-1)s(-1), which can be inhibited by cyclosporin A with an IC50 value of 60nM. Using a polyclonal antibody against Cyp40 we have found that Cyp40 is predominantly cytoplasmic, and that its expression is induced 3-4-fold by heat shock. Moreover, Cyp40 can be coprecipitated from yeast extracts with the cytosolic molecular chaperone Hsp90. Surprisingly, a Cyp40-deficient yeast strain is fully viable at normal and elevated temperatures. Cyp40 is also dispensable for normal regulation of vertebrate steroid receptors in yeast. While other immunophilins could conceivably compensate a Cyp40 defect, our results are compatible with the notion that immunophilins may be fortuitous partners in the biochemically established steroid receptor-Hsp90 complex." @default.
- W2034143658 created "2016-06-24" @default.
- W2034143658 creator A5007056600 @default.
- W2034143658 creator A5045503547 @default.
- W2034143658 creator A5053394320 @default.
- W2034143658 date "1997-01-01" @default.
- W2034143658 modified "2023-09-25" @default.
- W2034143658 title "Functional Analysis of the Yeast 40 kDa Cyclophilin Cyp40 and Its Role for Viability and Steroid Receptor Regulation" @default.
- W2034143658 cites W116814209 @default.
- W2034143658 cites W1499287202 @default.
- W2034143658 cites W1552859356 @default.
- W2034143658 cites W1576929531 @default.
- W2034143658 cites W1580193095 @default.
- W2034143658 cites W1676827704 @default.
- W2034143658 cites W1811531272 @default.
- W2034143658 cites W1906465230 @default.
- W2034143658 cites W1967407965 @default.
- W2034143658 cites W1976582108 @default.
- W2034143658 cites W1979651801 @default.
- W2034143658 cites W1983323106 @default.
- W2034143658 cites W1983496641 @default.
- W2034143658 cites W1985898403 @default.
- W2034143658 cites W1988069951 @default.
- W2034143658 cites W1988864942 @default.
- W2034143658 cites W1991148544 @default.
- W2034143658 cites W1992593876 @default.
- W2034143658 cites W1998393335 @default.
- W2034143658 cites W1999465079 @default.
- W2034143658 cites W2001101609 @default.
- W2034143658 cites W2005174079 @default.
- W2034143658 cites W2007996847 @default.
- W2034143658 cites W2010533729 @default.
- W2034143658 cites W2016620115 @default.
- W2034143658 cites W2018390282 @default.
- W2034143658 cites W2020597127 @default.
- W2034143658 cites W2024695552 @default.
- W2034143658 cites W2036877456 @default.
- W2034143658 cites W2037551587 @default.
- W2034143658 cites W2038933991 @default.
- W2034143658 cites W2040385763 @default.
- W2034143658 cites W2043632152 @default.
- W2034143658 cites W2044406741 @default.
- W2034143658 cites W2044872880 @default.
- W2034143658 cites W2050584068 @default.
- W2034143658 cites W2051301930 @default.
- W2034143658 cites W2052203027 @default.
- W2034143658 cites W2055176898 @default.
- W2034143658 cites W2057948736 @default.
- W2034143658 cites W2067435429 @default.
- W2034143658 cites W2069965054 @default.
- W2034143658 cites W2070484979 @default.
- W2034143658 cites W2074654736 @default.
- W2034143658 cites W2079336387 @default.
- W2034143658 cites W2091966714 @default.
- W2034143658 cites W2092266686 @default.
- W2034143658 cites W2108794906 @default.
- W2034143658 cites W2109188826 @default.
- W2034143658 cites W2114858319 @default.
- W2034143658 cites W2119892511 @default.
- W2034143658 cites W2129366913 @default.
- W2034143658 cites W2149227294 @default.
- W2034143658 cites W2168700356 @default.
- W2034143658 cites W2169333620 @default.
- W2034143658 cites W2289619544 @default.
- W2034143658 cites W2404397406 @default.
- W2034143658 cites W4243484967 @default.
- W2034143658 cites W4244230354 @default.
- W2034143658 cites W4248992638 @default.
- W2034143658 cites W934392456 @default.
- W2034143658 doi "https://doi.org/10.1515/bchm.1997.378.5.381" @default.
- W2034143658 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9191025" @default.
- W2034143658 hasPublicationYear "1997" @default.
- W2034143658 type Work @default.
- W2034143658 sameAs 2034143658 @default.
- W2034143658 citedByCount "41" @default.
- W2034143658 countsByYear W20341436582013 @default.
- W2034143658 countsByYear W20341436582015 @default.
- W2034143658 countsByYear W20341436582018 @default.
- W2034143658 countsByYear W20341436582020 @default.
- W2034143658 countsByYear W20341436582023 @default.
- W2034143658 crossrefType "journal-article" @default.
- W2034143658 hasAuthorship W2034143658A5007056600 @default.
- W2034143658 hasAuthorship W2034143658A5045503547 @default.
- W2034143658 hasAuthorship W2034143658A5053394320 @default.
- W2034143658 hasConcept C104317684 @default.
- W2034143658 hasConcept C142724271 @default.
- W2034143658 hasConcept C154472170 @default.
- W2034143658 hasConcept C159654299 @default.
- W2034143658 hasConcept C170493617 @default.
- W2034143658 hasConcept C181199279 @default.
- W2034143658 hasConcept C185592680 @default.
- W2034143658 hasConcept C2775962898 @default.
- W2034143658 hasConcept C2776326521 @default.
- W2034143658 hasConcept C2777576037 @default.
- W2034143658 hasConcept C2779222958 @default.
- W2034143658 hasConcept C2781098309 @default.
- W2034143658 hasConcept C2781264208 @default.
- W2034143658 hasConcept C2910190745 @default.
- W2034143658 hasConcept C2910677434 @default.
- W2034143658 hasConcept C54355233 @default.