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• W2034143861 abstract "The use of the DNA-specific fluorochrome DAPI has been extended to stage assessment of fresh pollen in wheat and maize. Membrane permeabilization by Triton X-100 incorporated in the staining solution allows access of the fluorochrome to nuclear DNA. At all stages of gametophytic development, the nuclei can be sharply visualized. Starch does not interfere with the fluorochrome so that it is possible to study the second pollen grain mitosis and sperm differentiation. With its rapidity and reliability, this technique represents an efficient tool for routine staging or investigation of the nuclear status of the pollen grains." @default.
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• W2034143861 date "1987-01-01" @default.
• W2034143861 modified "2023-09-27" @default.
• W2034143861 title "Rapid Assessment of Microspore and Pollen Development Stage in Wheat and Maize Using Dapi and Membrane Permeabilization" @default.
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• W2034143861 doi "https://doi.org/10.3109/10520298709108014" @default.
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